Epizootic canine distemper virus infection among wild mammals
Epizootic canine distemper virus infection among wild mammals
复制标题
DOI：
10.1016/j.vetmic.2011.07.006
复制
发表时间：
2012-01-27
影响因子：
3.3
通讯作者：
Maeda, Ken
中科院分区：
文献类型：
--
作者：
Kameo, Yuki;Nagao, Yumiko;Maeda, Ken
2007年春天，日本和歌山县田边市附近捕获了7只浣熊狗和一只黄鼠狼。通过病毒分离、用抗犬瘟热病毒（CDV）多克隆抗体进行免疫染色和市售的CDV抗原检测试剂盒，鉴定捕获后1-2天动物死亡的病原体为犬瘟热病毒（CDV）。血凝素基因的序列分析表明，分离的病毒属于Asia-1基因型，其549位由酪氨酸（Y）变为组氨酸（H），这与犬瘟热病毒向非犬宿主的传播有关。然后在该地区的野生动物中进行了CDV的血清调查。试验动物包括浣熊104只、野猪41只、浣熊狗19只、西卡鹿5只、獾2只、黄鼠狼1只、貂1只、西伯利亚黄鼠狼1只、狐狸1只。病毒中和试验表明，除狐狸和黄鼬外，所有被测动物均具有抗犬瘟热病毒的中和抗体。有趣的是，检测的41只野猪中有11只（27%）和5只西卡鹿中的2只具有CDV VN抗体。这些结果表明，CDV感染是广泛的野生哺乳动物在这次流行病。(C)2011 Elsevier B. V.保留所有权利。
In the spring of 2007, seven raccoon dogs and a weasel were captured near the city of Tanabe in Wakayama prefecture, Japan. The causative agent of the animals' death 1-2 days after capture was identified as canine distemper virus (CDV) by virus isolation, immunostaining with an anti-CDV polyclonal antibody, and a commercially available CDV antigen-detection kit. Sequence analysis of hemagglutinin genes indicated the isolated viruses belong to genotype Asia-1 and possess the substitution from tyrosine (Y) to histidine (H) at position 549 that is associated with the spread of CDV to non-canine hosts. A serosurvey for CDV was then conducted among wild animals in the region. The animals assayed consisted of 104 raccoons, 41 wild boars, 19 raccoon dogs, five Sika deer, two badgers, one weasel, one marten, one Siberian weasel and one fox. Virus-neutralization (VN) tests showed that, except for fox and weasel, all of the species assayed had VN antibodies to CDV. Interestingly, 11 of the 41 wild boars (27%) and two of the five Sika deer assayed possessed VN antibodies to CDV. These findings indicate that CDV infection was widespread among wild mammals during this epizootic. (C) 2011 Elsevier B.V. All rights reserved.