Identification and structural elucidation of steroidal saponins from the root of Paris polyphylla by HPLC-ESI-QTOF-MS/MS
Identification and structural elucidation of steroidal saponins from the root of Paris polyphylla by HPLC-ESI-QTOF-MS/MS
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HPLC-ESI-QTOF-MS/MS 鉴定重楼甾体皂苷及结构解析
DOI：
10.1080/14786419.2015.1007137
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发表时间：
2015-10-02
影响因子：
2.2
通讯作者：
Liao, Liang
中科院分区：
文献类型：
--
作者：
Ling, Yun;Fu, Zhiwen;Liao, Liang
重楼根(RPP)具有清热解毒、消肿、镇静、止痛、止血等功效，长期以来被广泛用作中药。为了阐明生物活性物质，确保临床用药的安全性，建立了高效液相色谱-电喷雾电离-四极杆飞行时间-质谱仪联用技术(HPLC-ESI-QTOF-MS/MS)，建立了一种可行、准确的方法学。采用Agilent Poroshell120EC-C18色谱柱(2.7×100 mm，i.d.，2.7μm)，以0.1%甲酸水溶液和乙腈为流动相，梯度洗脱。根据所提出的策略，鉴定或初步鉴定了30个化合物，其中主要包括甾体皂苷，其中2个为首次报道为RPP中潜在的新的甾体皂苷。综上所述，高效液相色谱-电喷雾飞行时间-质联用联用技术是分离和鉴定植物提取物中甾体皂苷的一种可行、可靠的方法。
The root of Paris polyphylla (RPP) is widely used as a traditional Chinese medicine for a long time due to the good properties of heat-clearing and detoxicating, detumescence, sedation, acesodyne and haemostasis. To clarify on the bioactive substances and ensure the safety in clinical medication, a feasible and accurate strategy was developed by applying the high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS). Separation was performed an Agilent poroshell 120 EC-C18 column (2.7 × 100 mm, i.d., 2.7 μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Based on the proposed strategy, 30 constituents, mainly including steroidal saponins, were characterised or tentatively identified, 2 of which were the first to be reported as the potential new steroidal saponins in RPP. In conclusion, the HPLC-ESI-QTOF-MS/MS is a feasible and credible technique to separate and identify steroidal saponins from botanical extracts.