The characterization of testicular cell (TC)‐specific T‐cell clones induced by intratesticular Listeria monocytogenes infection: TC‐specific T cells with atypical cytokine profile transfer orchitis
The characterization of testicular cell (TC)‐specific T‐cell clones induced by intratesticular Listeria monocytogenes infection: TC‐specific T cells with atypical cytokine profile transfer orchitis
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睾丸内单核细胞增生李斯特菌感染诱导的睾丸细胞 (TC) 特异性 T 细胞克隆的特征：具有非典型细胞因子谱转移性睾丸炎的 TC 特异性 T 细胞
DOI：
10.1046/j.1365-2567.1997.00306.x
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发表时间：
1997
期刊：
影响因子：
6.4
通讯作者：
K. Nomoto
中科院分区：
文献类型：
--
作者：
G. Matsuzaki;K. Sonoda;A. Mukasa;H. Yamada;T. Nakamura;H. Ikebe;S. Hamano;K. Nomoto
单核细胞增生李斯特菌单侧感染小鼠睾丸不仅诱导李斯特菌特异性T细胞，而且诱导自身反应性T细胞，其可以将实验性自身免疫性睾丸炎（EAO）转移到幼稚小鼠中。 为了研究自身反应性T细胞的特征，我们从睾丸内感染小鼠的脾脏中建立了6个睾丸细胞（TC）特异性T细胞克隆。 所有克隆均表达CD 4和T细胞受体（TCR）αβ，6个克隆中有4个表达Vβ8。它们在存在同源脾抗原呈递细胞的情况下对TC显示出增殖反应，但对李斯特菌抗原（Ag）没有交叉反应。当用TC刺激时，它们产生干扰素-γ（IFN-γ），但不能检测到白细胞介素-2（IL-2）、IL-4和IL-10。即使在没有Ag和IL-2的情况下进行10天的静息培养后用TC重新刺激，也没有检测到IL-2的产生，尽管它们在重新刺激培养中增殖。即使在存在抗IL-2 mAb的情况下，TC特异性T细胞克隆也显示出对TC的增殖反应。 观察结果表明，TC特异性产生IFN-γ的T细胞在不存在自分泌的情况下增殖。 静脉内和睾丸内注射这些克隆转移EAO在同基因的幼稚小鼠。这些结果表明L.睾丸中的单核细胞增多症感染诱导自身反应性或几丁质性CD 4 + T细胞，而不与细菌Ag交叉反应。 此外，这些数据表明，具有非典型细胞因子谱的CD 4 + T细胞可以有效地引起EAO。
A unilateral infection of Listeria monocytogenes into the testis of mice induces not only Listeria‐specific T cells but also autoreactive T cells that can transfer experimental autoimmune orchitis (EAO) into naive mice. To investigate the characteristics of the autoreactive T cells, we established six testicular cell (TC)‐specific T‐cell clones from the spleen of the intratesticularly infected mice. All the clones expressed CD4 and T‐cell receptor (TCR) αβ, and four of the six clones expressed Vβ8. They showed proliferative response to TC in the presence of syngeneic spleen antigen‐presenting cells, but did not cross‐react to Listeria antigen (Ag). They produced interferon‐γ (IFN‐γ) when stimulated with TC, but interleukin‐2 (IL‐2), IL‐4 and IL‐10 were undetectable. IL‐2 production was not detected even when they were restimulated with TC after a 10‐day resting culture without Ag and IL‐2, although they proliferated in the restimulation culture. Even in the presence of anti‐IL‐2 mAb, the TC‐specific T‐cell clones showed proliferative response against TC. The observations indicate that the TC‐specific IFN‐γ‐producing T cells proliferate in the absence of autocrine. Both intravenous and intratesticular injection of these clones transferred EAO in syngeneic naive mice. These results suggest that L. monocytogenes infection in the testis induces autoreactive orchitogenic CD4+ T cells without cross‐reactivity to bacterial Ag. Furthermore, these data demonstrate that CD4+ T cells with an atypical cytokine profile can efficiently cause EAO.