Effect of p-nitrophenyl-beta-D-xyloside on proteoglycan and glycosaminoglycan biosynthesis in rat serosal mast cell cultures.
Effect of p-nitrophenyl-beta-D-xyloside on proteoglycan and glycosaminoglycan biosynthesis in rat serosal mast cell cultures.
复制标题
对硝基苯基-β-D-木糖苷对大鼠浆膜肥大细胞培养物中蛋白聚糖和糖胺聚糖生物合成的影响。
DOI：
10.1016/s0021-9258(19)68354-7
复制
发表时间：
1982
影响因子：
4.8
通讯作者：
Frank Austen
中科院分区：
文献类型：
--
作者：
Richard L. Stevens;K.;Frank Austen
在热灭活胎牛血清存在下培养的大鼠血清肥大细胞在3小时脉冲和2小时追踪后将（35 S）硫酸盐掺入约Mr = 750，000的肝素蛋白聚糖中。β-D-木糖苷（0.1mM）处理大鼠肥大细胞培养物导致总（35 S）硫酸盐掺入的不显著增加和游离糖胺聚糖的出现，而蛋白聚糖大小没有变化。在较高的β-D-木糖苷浓度下，总（35 S）硫酸盐掺入被抑制，并且观察到相对糖胺聚糖含量的增加伴随着蛋白聚糖量和大小的减少。通过对软骨素酶ABC消化、亚硝酸水解、[3 H]己糖胺含量和电泳迁移率的敏感性评估，在所有培养物中，只有肝素链聚合到蛋白聚糖核心上。相反，仅在β-D-木糖苷处理后出现的单个糖胺聚糖主要是硫酸软骨素而不是肝素，表明β-D-木糖苷受体支持硫酸软骨素而不是肝素糖胺聚糖的聚合。因此，肽核心是大鼠腹腔肥大细胞合成肝素糖胺聚糖的重要决定因素。
Rat serosal mast cells cultured in the presence of heat-inactivated fetal calf serum incorporated (35S) sulfate into heparin proteoglycan of approximately Mr = 750,000 after a 3-h pulse and a 2-h chase. beta-D-Xyloside (0.1 mM) treatments of cultures of rat mast cells resulted in an insignificant increase in total (35S) sulfate incorporation and the appearance of free glycosaminoglycans without a change in proteoglycan size. At higher beta-D-xyloside concentrations, total (35S)sulfate incorporation was inhibited and an increase in the relative glycosaminoglycan content was observed concomitant with a reduction in proteoglycan amount and size. As assessed by susceptibility to digestion by chondroitinase ABC, hydrolysis by nitrous acid, [3H]hexosamine content, and electrophoretic mobility, only heparin chains were polymerized onto the proteoglycan core in all cultures. In contrast, individual glycosaminoglycans which appeared only after beta-D-xyloside treatment were predominantly chondroitin sulfate rather than heparin, indicating that the beta-D-xyloside acceptor supported polymerization of chondroitin sulfate but not of heparin glycosaminoglycan. Thus, the peptide core is an important determinant for the synthesis of heparin glycosaminoglycan by rat peritoneal mast cells.