PAX8 Expression in the Crystalline Lens and Lens-Derived Lesions.
PAX8 Expression in the Crystalline Lens and Lens-Derived Lesions.
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DOI：
10.1016/j.xops.2021.100024
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发表时间：
2021-06
影响因子：
--
通讯作者：
Eagle Jr, Ralph C.
中科院分区：
文献类型：
--
作者：
Milman, Tatyana;Mudhar, Hardeep Singh;Eagle Jr, Ralph C.
关键词：
通过免疫组织化学评估 PAX8 在正常儿童和成人晶状体中的表达，并评估 PAX8 免疫组织化学染色在诊断晶状体起源的形态学挑战性病变中的有用性。回顾性、观察性病例系列。 14 个先天性和后天性晶状体源性病变和 10 个对照晶状体。对所有组织进行苏木精-伊红和高碘酸-希夫染色以及 PAX8、波形蛋白、S100、平滑肌肌动蛋白、AE1/AE3、细胞角蛋白 7 和细胞角蛋白 5/6 抗体的免疫组织化学检测。 PAX8 表达在正常晶状体和晶状体衍生病变中的分布。记录检索确定了 10 个正常儿童和成人晶状体、1 个晶状体瘤、1 个患有粘连性白内障的 Peters 异常、1 个患有先天性锥体白内障形成的晶状体囊、2 个患有前部和后部囊下白内障形成的晶状体、3 个术后白内障晶状体（Soemmerring 环白内障和囊膜纤维化）以及 6 个包含各种化生成分的角膜后膜角膜内皮、化生晶状体上皮、角膜基质和上皮向下生长。在正常儿童和成人晶状体的晶状体上皮和赤道晶状体弓中观察到强的核PAX8表达。在保留一些上皮形态特征的病变中也观察到核PAX8表达，例如斑状细胞瘤、粘连性白血病、先天性锥体白内障和晶状体上皮分化的眼内膜成分。 PAX8表达在经历间质转化的晶状体上皮病变中丢失，例如前囊下白内障和囊膜纤维化。 PAX8 抗体可能是免疫组织化学组的有用辅助物，用于保留上皮形态特征的形态上具有挑战性的晶状体上皮衍生病变。 PAX8 对于诊断以上皮-间质转化为特征的晶状体源性病变没有用处。
To evaluate PAX8 expression by immunohistochemistry in the normal pediatric and adult crystalline lens and to assess the usefulness of PAX8 immunohistochemical stain in the diagnosis of morphologically challenging lesions of lenticular origin. Retrospective, observational case series. Fourteen congenital and acquired lens-derived lesions and 10 control crystalline lenses. Hematoxylin–eosin and periodic acid–Schiff stains and an immunohistochemical panel of PAX8, vimentin, S100, smooth muscle actin, AE1/AE3, cytokeratin 7, and cytokeratin 5/6 antibodies were performed on all tissues. Distribution of PAX8 expression in normal crystalline lens and in lens-derived lesions. Records search identified 10 normal pediatric and adult crystalline lenses, 1 phakomatous choristoma, 1 Peters anomaly with adherent leukoma, 1 lens capsule with congenital pyramidal cataract formation, 2 lenses with anterior and posterior subcapsular cataract formation, 3 postsurgical cataractous lenses (Soemmerring ring cataract and capsular fibrosis), and 6 retrocorneal membranes that incorporated various components of metaplastic corneal endothelium, metaplastic lens epithelium, corneal stroma, and epithelial downgrowth. Strong nuclear PAX8 expression was observed in the lens epithelium and in the equatorial lens bow of normal pediatric and adult lenses. Nuclear PAX8 expression also was observed in the lesions that retained some of the epithelial morphologic features, such as phakomatous choristoma, adherent leukoma, congenital pyramidal cataract, and components of intraocular membranes with lens epithelial differentiation. PAX8 expression was lost in lens epithelial lesions that had undergone mesenchymal transition, such as anterior subcapsular cataract and capsular fibrosis. PAX8 antibody may be a useful adjunct to the immunohistochemical panels in morphologically challenging lens epithelial-derived lesions that retain epithelial morphologic features. PAX8 is not useful in the diagnosis of lens-derived lesions that feature epithelial–mesenchymal transition.
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影响因子：
4.2
作者：
Jakobiec, Frederick A.;Bhat, Pooja
通讯作者：
Bhat, Pooja
影响因子：
4.4
作者：
Pedersen, Hilde R.;Baraas, Rigmor C.;Neitz, Maureen
通讯作者：
Neitz, Maureen
影响因子：
2.7
作者：
Davis, Noa;Yoffe, Chen;Ashery-Padan, Ruth
通讯作者：
Ashery-Padan, Ruth
影响因子：
--
作者：
Sels, Lise;Dirven, Werner;Leys, Anita
通讯作者：
Leys, Anita
DOI：
10.1034/j.1600-0420.1999.770320.x
发表时间：
1999-06-01
期刊：
ACTA OPHTHALMOLOGICA SCANDINAVICA
影响因子：
--
作者：
Seregard, S
通讯作者：
Seregard, S