MYB Transcription Factors in Chinese Pear (Pyrus bretschneideri Rehd.): Genome-Wide Identification, Classification, and Expression Profiling during Fruit Development.
MYB Transcription Factors in Chinese Pear (Pyrus bretschneideri Rehd.): Genome-Wide Identification, Classification, and Expression Profiling during Fruit Development.
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DOI：
10.3389/fpls.2016.00577
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发表时间：
2016
影响因子：
5.6
通讯作者：
Cai Y
中科院分区：
文献类型：
--
作者：
Cao Y;Han Y;Li D;Lin Y;Cai Y
MYB家族是植物中最大的转录因子家族之一。虽然已有研究表明MYB家族在梨的次生代谢中发挥重要作用，但目前对中国梨MYB家族的研究还不多见。已被报告。本研究对中国梨MYB基因（命名为PbMYBs）进行了全基因组分析，包括其亲缘关系、结构、染色体定位、启动子区、GO注释和共线性分析。在梨基因组中共鉴定出129个PbMYB基因，系统发育分析将其分为31个亚组。这些PbMYB不均匀分布在16条染色体（共17条染色体）中。基因重复事件的发生表明，全基因组重复和片段重复可能在PbMYB基因家族的扩展中发挥了关键作用。Ka/Ks分析表明，PbMYBs重复后主要经历了纯化选择和限制性功能分化。种间微共线性分析显示梨和桃之间的同源性最大，其次是李和草莓。随后，20 PbMYB基因，可能参与木质素的生物合成，根据其系统发育关系的表达模式进行了检查整个果实发育。在检测的20个基因中，PbMYB 25和PbMYB 52表现出与先前报道的木质素含量的典型变化一致的表达模式。此外，亚细胞定位分析表明，两个蛋白PbMYB 25和PbMYB 52定位于细胞核。PbMYB 25和PbMYB 52可能是梨果实发育过程中木质素生物合成调控的候选基因。本研究为梨MYB基因家族的功能研究提供了有用的信息。
The MYB family is one of the largest families of transcription factors in plants. Although, some MYBs were reported to play roles in secondary metabolism, no comprehensive study of the MYB family in Chinese pear (Pyrus bretschneideri Rehd.) has been reported. In the present study, we performed genome-wide analysis of MYB genes in Chinese pear, designated as PbMYBs, including analyses of their phylogenic relationships, structures, chromosomal locations, promoter regions, GO annotations, and collinearity. A total of 129 PbMYB genes were identified in the pear genome and were divided into 31 subgroups based on phylogenetic analysis. These PbMYBs were unevenly distributed among 16 chromosomes (total of 17 chromosomes). The occurrence of gene duplication events indicated that whole-genome duplication and segmental duplication likely played key roles in expansion of the PbMYB gene family. Ka/Ks analysis suggested that the duplicated PbMYBs mainly experienced purifying selection with restrictive functional divergence after the duplication events. Interspecies microsynteny analysis revealed maximum orthology between pear and peach, followed by plum and strawberry. Subsequently, the expression patterns of 20 PbMYB genes that may be involved in lignin biosynthesis according to their phylogenetic relationships were examined throughout fruit development. Among the 20 genes examined, PbMYB25 and PbMYB52 exhibited expression patterns consistent with the typical variations in the lignin content previously reported. Moreover, sub-cellular localization analysis revealed that two proteins PbMYB25 and PbMYB52 were localized to the nucleus. All together, PbMYB25 and PbMYB52 were inferred to be candidate genes involved in the regulation of lignin biosynthesis during the development of pear fruit. This study provides useful information for further functional analysis of the MYB gene family in pear.