Enrichment and Purification of Total Flavonoid C-Glycosides from Abrus mollis Extracts with Macroporous Resins
Enrichment and Purification of Total Flavonoid C-Glycosides from Abrus mollis Extracts with Macroporous Resins
复制标题
大孔树脂富集纯化相思子提取物中总黄酮C-苷
DOI：
10.1021/ie3004094
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发表时间：
2012-05-30
影响因子：
4.2
通讯作者：
Li, Ping
中科院分区：
文献类型：
--
作者：
Du, Huibin;Wang, Hao;Li, Ping
本研究评价了6种大孔树脂富集纯化鸭茅提取物中总黄酮c -苷类化合物（vicenin- 2,1；异麦草苷，2；麦草苷，3）的性能和分离特性。研究了总黄酮c -苷在HPD-100、HPD-450、AB-8、HPD-600、DA-201、HPD-417等大孔树脂上的吸附解吸性能。结果表明，在6种树脂中，HPD-100树脂对这3种黄酮类c -苷的吸附和解吸能力最好。在HPD-100树脂上构建吸附等温线，符合Langmuir （R2 > 0.99）和Freundlich （R2 > 0.89, 0.1 < 1/n < 0.5）模型。经HPD-100树脂填充层析柱梯度洗脱后，黄酮类化合物c -苷（1、2、3）的含量分别从提取液的2.05、2.90、2.30%提高到14.3、21.9、15.1%。化合物1、2、…
In the present study, the performance and separation characteristics of six macroporous resins for the enrichment and purification of total flavonoid C-glycosides (vicenin-2, 1; isoschaftoside, 2; schaftoside, 3) from Abrus mollis extracts have been evaluated. The adsorption and desorption properties of total flavonoid C-glycosides were studied on macroporous resins, including HPD-100, HPD-450, AB-8, HPD-600, DA-201, and HPD-417. According to the results, HPD-100 resin showed the best adsorption and desorption capacity for these three flavonoid C-glycosides among six resins. Adsorption isotherms were constructed on HPD-100 resin and fitted well to the Langmuir (R2 > 0.99) and Freundlich (R2 > 0.89, 0.1 < 1/n < 0.5) models. After the treatment with gradient elution on HPD-100 resin packed chromatography column, the contents of the flavonoid C-glycosides (1, 2, and 3) increased from 2.05, 2.90, and 2.30% in the extracts to 14.3, 21.9, and 15.1%, respectively, in the product. The recoveries of compounds 1, 2...