K-ras mutation in adenomas and carcinomas of the ampulla of vater.
K-ras mutation in adenomas and carcinomas of the ampulla of vater.
复制标题
壶腹腺瘤和癌中的 K-ras 突变。
作者：
J. Howe;D. Klimstra;C. Cordon;P. Paty;P. Park;M. Brennan
K-ras 突变在 Vater 壶腹肿瘤进展中的作用尚不清楚。为了研究壶腹肿瘤中 K-ras 突变的频率和时间，从 96 个切除肿瘤的石蜡块上显微解剖浸润性癌和邻近腺瘤的区域。提取DNA，进行K-ras外显子1的PCR扩增，并对PCR产物进行测序。使用chi2检验、对数秩检验和Cox比例风险模型对K-ras突变与患者生存和临床病理因素相关的统计分析。 92 例壶腹癌中的 34 例 (37.0%) 和 46 例腺瘤中的 25 例 (54.3%) 存在 K-ras 外显子 1 突变。与具有 K-ras 突变的癌相邻的 23 例腺瘤中，有 22 例 (95.7%) 也存在 K-ras 突变。与 K-ras 突变显着相关的唯一临床病理因素是肿瘤大小 > 2 cm (P = = 0.035)。患者生存率与 K-ras 突变状态无关（P = 0.31）。我们得出结论，K-ras 突变在 Vater 壶腹部腺瘤和癌中都很常见，并且似乎作为早期遗传事件发生。突变谱与结直肠肿瘤中观察到的突变谱相似，并且这些突变与患者生存率没有显着相关性。
The role of K-ras mutations in the progression of tumors of the ampulla of Vater is not well understood. To study the frequency and timing of K-ras mutations in ampullary tumors, areas of invasive carcinoma and adjacent adenomas were microdissected from paraffin blocks from 96 resected tumors. DNA was extracted, PCR amplification of K-ras exon 1 was performed, and PCR products were sequenced. Statistical analysis of K-ras mutations with respect to patient survival and clinicopathological factors was performed using the chi2 test, log-rank test, and Cox proportional hazard model. Thirty-four of 92 ampullary carcinomas (37.0%) and 25 of 46 adenomas (54.3%) had mutations in K-ras exon 1. Twenty-two of 23 (95.7%) adenomas adjacent to carcinomas with K-ras mutations also had K-ras mutations. The only clinicopathological factor significantly associated with K-ras mutation was tumor size >2 cm (P = = 0.035). Patient survival did not correlate with the K-ras mutation status (P = 0.31). We conclude that K-ras mutations are frequent in both adenomas and carcinomas of the ampulla of Vater and appear to occur as an early genetic event. The spectrum of mutations is similar to that observed in colorectal neoplasms, and these do not significantly correlate with patient survival.
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影响因子：
10.4
作者：
Capella G;Cronauer-Mitra S;Pienado MA;Perucho M
通讯作者：
Perucho M
影响因子：
--
作者：
S. Finkelstein;R. Przygodzki;V. Pricolo;R. Sayegh;A. Bakker;P. Swalsky;G. Keller
通讯作者：
S. Finkelstein;R. Przygodzki;V. Pricolo;R. Sayegh;A. Bakker;P. Swalsky;G. Keller
检测人胰腺腺癌细针抽吸物中的 c-K-ras 突变。
影响因子：
11.2
作者：
Shibata,D;Almoguera,C;Forrester,K;Dunitz,J;Martin,SE;Cosgrove,MM;Perucho,M;Arnheim,N
通讯作者：
Arnheim,N
DOI：
10.1073/pnas.86.7.2403
发表时间：
1989-04-01
影响因子：
11.1
作者：
BURMER, GC;LOEB, LA
通讯作者：
LOEB, LA
影响因子：
158.5
作者：
VOGELSTEIN, B;FEARON, ER;BOS, JL
通讯作者：
BOS, JL