Dynamics in serum of the inflammatory markers serum amyloid A (SAA), haptoglobin, fibrinogen and alpha2-globulins during induced noninfectious arthritis in the horse.
Dynamics in serum of the inflammatory markers serum amyloid A (SAA), haptoglobin, fibrinogen and alpha2-globulins during induced noninfectious arthritis in the horse.
复制标题
马诱导非感染性关节炎期间血清中炎症标志物血清淀粉样蛋白 A (SAA)、触珠蛋白、纤维蛋白原和 α2 球蛋白的动态。
DOI：
--
复制
发表时间：
2010
影响因子：
2.2
通讯作者：
M. Forsberg
中科院分区：
文献类型：
--
作者：
C. Hultén;U. Grönlund;J. Hirvonen;R. Tulamo;M. Suominen;G. Marhaug;M. Forsberg
尽管非感染性关节疾病在马医学中很重要，但如果非感染性关节炎的局部炎症过程足够强烈，则可能引发的急性时相反应知之甚少，因此，本研究的目的是通过研究急性时相蛋白血清淀粉样蛋白A(SAA)、结合珠蛋白、纤维蛋白原和α2-球蛋白的动态变化来监测实验诱导的非感染性关节炎过程中的全身炎症反应。24匹健康标准种马，年龄3~7岁，经临床、放射学、血液学和血清生化检查证实健康。在诱导关节炎前(0h)、诱导后8、16、24、36和48h、诱导后3、4、5和15天，在右侧腕中关节内无菌注射两性霉素B。所有的马都出现了跛行，关节积液和关节发热，以及呼吸频率、心率和体温增加。24-36h后跛行开始减少，大多数动物在注射后第2天全身体征消失。在关节炎诱导后，急性期蛋白的浓度增加。SAA浓度从诱导后16h开始高于基线浓度，36~48h最高，为基线浓度的227倍。结合珠蛋白浓度从24 h开始高于基线浓度，48~96 h达最大值(1.14倍基线浓度)。纤维蛋白原最大浓度出现在注射后36-72小时，平均为基线浓度的0.87倍。从注射后24小时开始，纤维蛋白原浓度一直高于基线浓度。α2球蛋白浓度略有增加，是基线浓度的0.55倍。这些标记物在第15天恢复到基线浓度。我们的结果表明，两性霉素B诱导的单个关节的关节炎引起全身急性期反应，在病情的前两周，血清SAA、结合珠蛋白、纤维蛋白原和α2-球蛋白的浓度增加，因此，这种增加不一定是感染性关节炎的迹象。进一步的研究应该旨在确定慢性非传染性关节炎是否会导致血清中急性时相蛋白浓度的增加。
Despite the importance of noninfectious joint diseases in equine medicine, little is known about the acute phase response which may be elicited if the local inflammatory process of noninfectious arthritis is sufficiently strong, Therefore the aim of this study was to monitor the systemic inflammatory response during experimentally-induced noninfectious arthritis by studying the dynamics in serum of the acute phase proteins serum amyloid A (SAA), haptoglobin, fibrinogen and alpha2-globulins. Twenty-four Standardbred horses, age 3-7 years, found healthy on thorough clinical, radiological, haematological and serum biochemical examination, were injected aseptically into the right midcarpal joint with amphotericin B. Blood samples were drawn before induction of arthritis (0 h), and at 8, 16, 24, 36 and 48 h postinduction and then on Days 3, 4, 5 and 15 postinduction. All horses developed lameness with joint effusion and joint heat as well as increased respiratory rate, heart rate and body temperature. The lameness started to decline after 24-36 h and, in most animals, systemic signs disappeared on Day 2 postinjection. The concentration of the acute phase proteins increased following induction of arthritis. The SAA concentrations were higher than baseline concentrations from 16 h postinduction and were maximal at 36-48 h (227 times baseline concentration). The haptoglobin concentrations were higher than baseline concentrations from 24 h and were maximal at 48-96 h (1.14 times baseline concentration). The maximal concentrations of fibrinogen were seen between 36-72 h postinjection and increased on average 0.87 times from baseline concentrations. The fibrinogen concentrations were higher than baseline concentrations from 24 h postinjection. Alpha2-globulins concentrations showed a minor increase and increased 0.55 times from baseline concentrations. The markers had returned to baseline concentrations by Day 15. Our results demonstrate that amphotericin B-induced arthritis in a single joint gives rise to a systemic acute phase response measurable as increased concentrations in serum SAA, haptoglobin, fibrinogen and alpha2-globulins during the first 2 weeks of the condition and, thereby, that such an increase need not be indicative of infectious arthritis. Further research should be aimed at determining whether chronic noninfectious arthritis in the horse gives rise to increased acute phase protein concentrations in serum.
影响因子：
6.4
作者：
PETERSON, PK;GEKKER, G;SHARP, B
通讯作者：
SHARP, B