Search for primary infection by Pneumocystis carinii in a cohort of normal, healthy infants
Search for primary infection by Pneumocystis carinii in a cohort of normal, healthy infants
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DOI：
10.1086/319340
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发表时间：
2001-03-15
影响因子：
11.8
通讯作者：
Gigliotti, F
中科院分区：
文献类型：
--
作者：
Vargas, SL;Hughes, WT;Gigliotti, F
为了确定卡氏肺孢子虫是否与有能力宿主的临床疾病相关，107名正常健康婴儿参加了在智利进行的为期2年的前瞻性队列研究。通过卡氏肺孢子虫大亚基线粒体核糖体核糖核酸基因的特异性染色和巢式DNA扩增鉴定卡氏肺孢子虫。人型链球菌，并通过每2个月抽取的血清样品的酶联免疫吸附测定来评估血清转化。在74名样本可用于检测的婴儿中，24名（32%）在轻度呼吸道感染发作期间获得的鼻咽吸出物中鉴定出卡氏肺孢子虫DNA。24名婴儿中的3名（12.5%）与卡氏肺孢子虫检测阴性的50名婴儿中的0名相比，有呼吸暂停发作。在20个月大时仍留在研究中的79名婴儿中，有67名（85%）发生血清转化，14名（20.8%）在没有任何疾病症状的情况下发生血清转化。该研究表明，卡氏肺孢子虫DNA可以在健康婴儿中频繁检测到，并提出了他们可能是社区中卡氏肺孢子虫感染宿主的假设。需要进一步研究以确定卡氏肺孢子虫是否会导致婴儿明显的呼吸道疾病。
To determine whether Pneumocystis carinii is associated with clinical illness in the competent host, 107 normal, healthy infants were enrolled in a 2-year prospective cohort study in Chile. P. carinii was identified by specific stains and nested-deoxyribonucleic acid (DNA) amplification of the large subunit mitochondrial ribosomal ribonucleic acid gene of P. carinii f. sp. hominis, and seroconversion was assessed by enzyme-linked immunosorbent assay of serum samples drawn every 2 months. P. carinii DNA was identified in nasopharyngeal aspirates obtained during episodes of mild respiratory infection in 24 (32%) of 74 infants from whom specimens were available for testing. Three (12.5%) of those 24 infants versus 0 of 50 infants who tested negative for P. carinii had apnea episodes. Seroconversion developed in 67 (85%) of 79 infants who remained in the study by 20 months of age and occurred in the absence of any symptoms of disease in 14 (20.8%). The study indicates that P. carinii DNA can be frequently detected in healthy infants, and it raises the hypothesis that they may be an infectious reservoir of P. carinii in the community. Further investigation is needed to identify whether P. carinii causes overt respiratory disease in infants.