Identification of Leaf Rust Resistance Genes in Wheat Cultivars Produced in Kazakhstan
Identification of Leaf Rust Resistance Genes in Wheat Cultivars Produced in Kazakhstan
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DOI：
10.1556/0806.43.2015.056
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发表时间：
2016-06-01
影响因子：
1.6
通讯作者：
Purnhauser, L.
中科院分区：
文献类型：
--
作者：
Kokhmetova, A.;Madenova, A.;Purnhauser, L.
叶锈病是由叶锈菌（Puccinia recondita f.）小麦赤霉病是哈萨克斯坦小麦的主要病害之一。为了有效利用抗叶锈病基因，了解现有品种的抗病基因型是育种工作者的重要任务。在这项研究中，30个冬小麦条目生长和/或生产在哈萨克斯坦进行了调查，使用分子标记，以确定是否存在8个重要的Lr基因。这些基因型的分子筛选显示这些基因的频率存在对比差异。在30份供试材料中，17份含有Lr 1基因，6份含有Lr 26和Lr 34基因，3份含有Lr 10和Lr 37基因。两个品种分别携带Lr 19和Lr 68，而Lr 9在本研究的任何基因型中均未检测到。田间评价表明，两个最常见的两个基因（Lr 1和Lr 26）是无效的。虽然Lr 34提供了一定的保护，其余有效的Lr基因只发现在少数基因型：Lr 37发生在哈萨克基因型L-1090和Krasnopoliapadskaya 210和美国品种马德森; Lr 19和Lr 68可能只存在于俄罗斯和哈萨克品种，Pallada和叶格曼，分别。在三年的叶锈病试验中，哈萨克品种Karasay、Krasnopirapadskaya 210、L-1090、Arap和Yegmen、国外品种Madsen、Pallada和对照Parula（Lr 68）的抗性最高。这些数据可以帮助育种者将有效的Lr基因整合到新品种中。
Leaf rust, caused by Puccinia recondita f. sp. tritici, is one of the major diseases of wheat in Kazakhstan. To effectively use leaf rust resistance genes (Lr), it is important for breeders to know the resistance genotype in current cultivars. In this study, 30 winter wheat entries grown and/or produced in Kazakhstan were investigated using molecular markers to determine the presence and absence of eight important Lr genes. Molecular screening of these genotypes showed contrasting differences in the frequencies of these genes. Among the 30 entries, 17 carried leaf rust resistance gene Lr1, six had Lr26 and Lr34, and Lr10 and Lr37 were found in three cultivars. Two single cultivars separately carried Lr19 and Lr68, while Lr9 was not detected in any genotypes in this study. Field evaluation demonstrated that two of the most frequent two genes (Lr1 and Lr26) to be ineffective. While Lr34 provided some protection, the remaining effective Lr genes were found only in few genotypes: Lr37 occurred in Kazakh genotypes L-1090 and Krasnovodapadskaya 210 and in the US cultivar Madsen; Lr19 and Lr68 were likely present only in Russian and Kazakh cultivars, Pallada and Yegemen, respectively. The highest resistance over three years of leaf rust testing was found in Kazakh cultivars, Karasay, Krasnovodapadskaya 210, L-1090, Arap and Yegmen, foreign cultivars Madsen, Pallada and the control Parula (Lr68). Data may assist breeders to incorporate effective Lr genes into new cultivars.