MOLECULAR-CLONING OF THE CHROMOSOMAL BREAKPOINT OF B-CELL LYMPHOMAS AND LEUKEMIAS WITH THE T(11-14) CHROMOSOME-TRANSLOCATION
MOLECULAR-CLONING OF THE CHROMOSOMAL BREAKPOINT OF B-CELL LYMPHOMAS AND LEUKEMIAS WITH THE T(11-14) CHROMOSOME-TRANSLOCATION
复制标题
DOI：
10.1126/science.6610211
复制
发表时间：
1984-01-01
期刊：
影响因子：
56.9
通讯作者：
CROCE, CM
中科院分区：
文献类型：
--
作者：
TSUJIMOTO, Y;YUNIS, J;CROCE, CM
克隆了携带染色体11和14长臂易位的B细胞型慢性淋巴细胞白血病（CLL）细胞的染色体断裂点[t（11;14）（q13;q32）]。发现断裂点位于14号染色体易位长臂上人重链基因座的连接区段内。分离出对11号染色体特异的探针，该探针直接定位于14 q+染色体上的断裂点的5′端。该探针检测到亲代CLL细胞中同源基因组DNA片段的重排，并且还检测到来自具有t（11;14）易位的弥漫性大细胞淋巴瘤的DNA中的重排。这种重排的DNA片段不存在于伯基特淋巴瘤细胞与t（8;14）易位或在非肿瘤性人类淋巴母细胞样细胞。因此，该探针可用于鉴定和表征位于染色体11的q13带上的基因，该基因似乎参与携带t（11;14）易位的人B细胞的恶性转化。这个基因，命名为bcl-1，似乎与迄今为止描述的任何已知的逆转录病毒癌基因无关。
The chromosomal breakpoint of chronic lymphocytic leukemia (CLL) cells of the B-cell type carrying the translocated long arms of chromosomes 11 and 14 [t(11;14) (q13;q32)] was cloned. The breakpoint was found to be within the joining segment of the human heavy chain locus on the translocated long arm of chromosome 14. A probe that is specific for chromosome 11 and that maps immediately 5′ to the breakpoint on the 14q+chromosome was isolated. The probe detected a rearrangement of the homologous genomic DNA segment in the parental CLL cells and also in DNA from a diffuse large cell lymphoma with the t(11;14) translocation. This rearranged DNA segment was not present in Burkitt lymphoma cells with the t(8;14) translocation or in nonneoplastic human lymphoblastoid cells. The probe can thus be used to identify and characterize a gene located on band q13 of chromosome 11 that appears to be involved in the malignant transformation of human B cells carrying the t(11;14) translocation. This gene, namedbcl-1, appears to be unrelated to any of the known retrovirus oncogenes described to date.