Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule.
Molecular and cellular properties of PECAM-1 (endoCAM/CD31): a novel vascular cell-cell adhesion molecule.
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DOI：
10.1083/jcb.114.5.1059
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发表时间：
1991-09
影响因子：
7.8
通讯作者：
Newman, P J
中科院分区：
文献类型：
--
作者：
Albelda, S M;Muller, W A;Buck, C A;Newman, P J
PECAM-1是一种130-120 kD的完整膜糖蛋白，存在于血小板表面、培养的内皮细胞间连接和髓系细胞上。以前的研究表明，它是免疫球蛋白基因超家族的成员，并且针对这种蛋白的牛型抗体(Endocam)可以抑制内皮细胞与细胞的相互作用。这些数据提示PECAM-1可能作为一种血管细胞黏附分子发挥作用。通过将全长的PECAM-1基因导入细胞，进一步评价了该分子的功能。转基因COS7细胞、小鼠3T3细胞和L细胞在细胞表面表达130kD~120kD的糖蛋白，可与抗PECAM1多克隆和单抗反应。COS-7和3T3细胞转染体形成高度富含PECAM-1的细胞-细胞连接，这使人想起它在内皮细胞-细胞边界的分布。相反，该蛋白仍然弥漫地分布在PECAM-1转基因细胞的质膜中，这些细胞与模拟转染体接触。稳定表达PECAM-1的小鼠L细胞表现出钙依赖性聚集，这种聚集可被抗PECAM-1抗体抑制。这些结果表明，PECAM-1介导了细胞间的黏附，并支持它可能参与了血栓形成、伤口愈合和血管生成过程中发生的一些相互作用的事件。
PECAM-1 is a 130-120-kD integral membrane glycoprotein found on the surface of platelets, at endothelial intercellular junctions in culture, and on cells of myeloid lineage. Previous studies have shown that it is a member of the immunoglobulin gene superfamily and that antibodies against the bovine form of this protein (endoCAM) can inhibit endothelial cell-cell interactions. These data suggest that PECAM-1 may function as a vascular cell adhesion molecule. The function of this molecule has been further evaluated by transfecting cells with a full-length PECAM-1 cDNA. Transfected COS-7, mouse 3T3 and L cells expressed a 130-120-kD glycoprotein on their cell surface that reacted with anti-PECAM-1 polyclonal and monoclonal antibodies. COS-7 and 3T3 cell transfectants formed cell-cell junctions that were highly enriched in PECAM-1, reminiscent of its distribution at endothelial cell-cell borders. In contrast, this protein remained diffusely distributed within the plasma membrane of PECAM-1 transfected cells that were in contact with mock transfectants. Mouse L cells stably transfected with PECAM-1 demonstrated calcium-dependent aggregation that was inhibited by anti-PECAM antibodies. These results demonstrate that PECAM-1 mediates cell-cell adhesion and support the idea that it may be involved in some of the interactive events taking place during thrombosis, wound healing, and angiogenesis.