A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process
A continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process
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DOI：
10.1016/j.sna.2005.01.030
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发表时间：
2005-05-31
影响因子：
4.6
通讯作者：
Cho, YH
中科院分区：
文献类型：
--
作者：
Doh, I;Cho, YH
我们提出了一种采用流体动力介电(DEP)工艺的高通量连续细胞分离芯片。连续细胞分离芯片在分离通道中使用三个平面电极，其中正的DEP细胞被移出中心流线，而负的DEP细胞保留在中心流线中。在实验研究中，我们使用活的(活的)和死的(死的)酵母细胞的混合物来获得连续的细胞分离条件。在电场频率为5 MHz，介质电导率为5亩S/cm的条件下，制作的芯片以0.1~1亩L/分钟的变化流量对酵母细胞混合物进行连续分离，从而使活性酵母细胞和非活性酵母细胞的纯度分别为95.9-97.3和64.5-74.3%。该芯片在不同的混合流量下表现出恒定的细胞分离性能。(C)2005 Elsevier B.V.保留所有权利。
We present a high-throughput continuous cell separation chip using hydrodynamic dielectrophoresis (DEP) process. The continuous cell separation chip uses three planar electrodes in a separation channel, where the positive DEP cells are moved away from the central streamline while the negative DEP cells remain in the central streamline. In the experimental study, we use the mixture of viable (live) and nonviable (dead) yeast cells in order to obtain the continuous cell separation conditions. For the conditions of the electric fields frequency of 5 MHz and the medium conductivity of 5 mu S/cm, the fabricated chip performs a continuous separation of the yeast cell mixture at the varying flow-rate in the range of 0.1-1 mu l/min; thereby, resulting in the purity ranges of 95.9-97.3 and 64.5-74.3%, respectively, for the viable and nonviable yeast cells. The present chip demonstrates the constant cell separation performance for varying mixture flow-rates. (c) 2005 Elsevier B.V. All rights reserved.