Peanut- and cow's milk-specific IgE, Th2 cells and local anaphylactic reaction are induced in Balb/c mice orally sensitized with cholera toxin
Peanut- and cow's milk-specific IgE, Th2 cells and local anaphylactic reaction are induced in Balb/c mice orally sensitized with cholera toxin
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DOI：
10.1111/j.1398-9995.2005.00767.x
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发表时间：
2005-05-01
期刊：
影响因子：
12.4
通讯作者：
Wal, JM
中科院分区：
文献类型：
--
作者：
Adel-Patient, K;Bernard, H;Wal, JM
背景：建立特异性免疫球蛋白(Ig)E的动物模型，具有与人IgE相同的特异性和与过敏患者相似的临床症状，对于了解食物过敏的诱导和调节机制具有重要意义。注射明矾或灌胃霍乱毒素(CT)。监测WPPE特异性IgE、IgG1和IgG2a。检测IL-4、IL-5与干扰素-γ对再活化脾细胞的Th2细胞活性。通过检测粪便标本中的组胺来评价局部过敏反应。结果：BALB/c小鼠在注射花生后均能产生较高的IgE和IgG1反应。或口服致敏作用。在这两种情况下，抗体都是针对含有来自Ara h3/4的多肽的球蛋白片段的聚合物，并且对Ara H1和Ara H2的程度较小。IL-4和IL-5产生明显。BALB/c小鼠也可以对CMP致敏，表现为CMP特异性的IL-4和IL-5的分泌以及对整个酪蛋白、β-乳球蛋白、血清牛血清白蛋白和乳铁蛋白的特异性IgE的诱导。结论：与以往作者不同的是，Balb/c小鼠被致敏，并在口服花生或CMP加CT后出现过敏反应，为进一步研究免疫致病机制提供了一个有趣的模型。
Background: The development of animal models developing specific immunoglobulin (Ig)E presenting the same specificity as human IgE and similar clinical symptoms as those observed in allergic patients are of great interest for the understanding of mechanisms involved in the induction and regulation of food allergy.Methods: Balb/c female mice were sensitized with whole peanut protein extract (WPPE) by means of intraperitoneal (i.p.) injections with alum or gavages with cholera toxin (CT). The WPPE specific IgE, IgG1 and IgG2a were monitored. Th2 cells activation was analysed assaying interleukin (IL)-4 and IL-5 vs IFN gamma on reactivated splenocytes. Local anaphylactic reaction was evaluated by assaying histamine in faecal samples. The oral sensitization protocol was further extended to cow's milk proteins (CMP).Results: Balb/c mice developed high peanut-specific IgE and IgG1 responses either after i.p. or oral sensitizations. In both cases, antibodies were specific to polymer of glycinin fragments, containing polypeptides from Ara h3/4, and to a lesser extent to Ara h1 and Ara h2. Interleukin-4 and IL-5 production were evidenced. Balb/c mice could also be sensitized to CMP, as demonstrated by CMP-specific IL-4 and IL-5 secretions and induction of IgE specific for whole caseins, beta-lactoglobulin, serum bovine albumin and lactoferrin. Of interest was the occurrence of a local anaphylactic reaction in the peanut and CM models.Conclusions: In contrast with previous authors, Balb/c mice were sensitized and evidenced an allergic reaction after oral administrations of peanut or CMP plus CT, providing an interesting model for further studies on immunopathogenic mechanisms.