Rapid regulation of c-myc protooncogene expression by progesterone in the avian oviduct.
Rapid regulation of c-myc protooncogene expression by progesterone in the avian oviduct.
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DOI：
10.1073/pnas.85.6.1796
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发表时间：
1988-03
影响因子：
11.1
通讯作者：
K. Fink;E. Wieben;G. Woloschak;T. Spelsberg
中科院分区：
文献类型：
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作者：
K. Fink;E. Wieben;G. Woloschak;T. Spelsberg
已知受性类固醇调节的基因的mRNA水平在类固醇处理后1小时或更长时间才改变，尽管类固醇受体复合物在5分钟内结合到核受体位点。并分析c-myc表达。注射后10分钟，c-myc mRNA水平开始下降，以响应孕酮。mRNA水平继续下降，在30分钟时达到70%的下降，并在类固醇注射后8小时恢复到对照值。在这些相同的RNA制剂中，α-微管蛋白mRNA水平的变化明显较少。该效应取决于类固醇的剂量，并且具有靶组织特异性。这些变化发生得比卵清蛋白mRNA水平的变化快得多。单独的媒介物不改变c-myc mRNA水平。早期调控基因如c-myc可能代表类固醇受体在基因组中的初始作用位点。
The mRNA levels of genes known to be regulated by sex steroids are not altered until 1 hr or longer after steroid treatment, although the steroid receptor complexes are bound to nuclear acceptor sites within 5 min. In a search for early regulation of gene transcription, total chick oviduct RNA was isolated at various times after injection (i.p.) of progesterone and analyzed for c-myc expression. Levels of c-myc mRNA began to decrease in response to progesterone by 10 min after injection. The mRNA levels continued to decrease, reached a 70% reduction at 30 min, and returned to control values by 8 hr after steroid injection. Changes in alpha-tubulin mRNA levels were markedly less in these same RNA preparations. The effect was dependent on the dose of the steroid and was target-tissue specific. These changes occurred much more rapidly than changes in egg-white protein mRNA levels. Vehicle alone did not alter c-myc mRNA levels. Early regulated genes such as c-myc may represent the initial site of action of steroid receptors in the genome.