SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C-elegans embryos
SRC-1 and Wnt signaling act together to specify endoderm and to control cleavage orientation in early C-elegans embryos
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DOI：
10.1016/s1534-5807(02)00185-5
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发表时间：
2002-07-01
影响因子：
11.8
通讯作者：
Mello, CC
中科院分区：
文献类型：
--
作者：
Bei, YX;Hogan, J;Mello, CC
在早期C。在线虫胚胎中，后卵裂球P2和腹卵裂球EMS之间的信号传导指定内胚层并定向EMS细胞的分裂轴。虽然Wnt信号有助于这种极化的相互作用，没有突变体确定日期废除P2/EMS信号。在这里，我们表明，两个酪氨酸激酶相关的基因，src-1和MES-1，需要P2和EMS之间的磷酸酪氨酸的积累。此外，src-1和mes-1突变体强烈增强与Wnt途径突变体相关的内胚层和EMS纺锤体旋转缺陷。SRC-1和MES-1双向发信号以控制EMS和P2中的细胞命运和分裂方向。我们的研究结果表明，Wnt和Src信号功能平行控制发育的结果在一个单一的响应细胞。
In early C. elegans embryos, signaling between a posterior blastomere, P2, and a ventral blastomere, EMS, specifies endoderm and orients the division axis of the EMS cell. Although Wnt signaling contributes to this polarizing interaction, no mutants identified to date abolish P2/EMS signaling. Here, we show that two tyrosine kinase-related genes, src-1 and mes-1, are required for the accumulation of phosphotyrosine between P2 and EMS. Moreover, src-1 and mes-1 mutants strongly enhance endoderm and EMS spindle rotation defects associated with Wnt pathway mutants. SRC-1 and MES-1 signal bidirectionally to control cell fate and division orientation in both EMS and P2. Our findings suggest that Wnt and Src signaling function in parallel to control developmental outcomes within a single responding cell.