Influence of Pancreas Preservation on Human Islet Isolation Outcomes: Impact of the Two-Layer Method
Influence of Pancreas Preservation on Human Islet Isolation Outcomes: Impact of the Two-Layer Method
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DOI：
10.1097/01.tp.0000133515.37892.d5
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发表时间：
2004-07
期刊：
影响因子：
6.2
通讯作者：
T. Tsujimura;Y. Kuroda;J. Avila;T. Kin;J. Oberholzer;A. M. Shapiro;J. Lakey
中科院分区：
文献类型：
--
作者：
T. Tsujimura;Y. Kuroda;J. Avila;T. Kin;J. Oberholzer;A. M. Shapiro;J. Lakey
背景资料。与全胰腺移植相比，人胰腺在胰岛移植中的保存面临更多的挑战和考虑。这项研究的目的是阐明威斯康星大学(UW)溶液的局限性和两层法(TLM)在分离人胰岛之前保存胰腺的潜力。方法：研究方法。我们回顾评估了2001年1月至2003年2月间的人类胰岛隔离记录。取自身体供体的人胰腺组织142例，分别用UW液和TLM液保存。使用标准方案进行人胰岛分离，并通过胰岛恢复和体外胰岛功能进行评估。结果。在UW液中冷缺血8-10小时是成功分离胰岛的关键。在UW液中冷藏10小时以上，很难从人胰腺中回收足够数量的可供移植的胰岛。TLM组总的胰岛恢复率明显高于UW组。冷藏10~16h后，TLM组胰岛分离成功率维持在62%，而UW组降至22%。TLM组移植的胰岛在受体体内效果良好。结论。在分离人类胰岛之前，使用UW液保存胰腺是有时间限制的。TLM是延长成功分离胰岛的最佳冷藏时间的一种潜在方法。
Background. Human pancreas preservation for islet transplantation holds additional challenges and considerations compared with whole pancreas transplantation. The purpose of this study was to clarify the limitations of the University of Wisconsin (UW) solution and the potentials of the two-layer method (TLM) for pancreas preservation before human islet isolation. Methods. We retrospectively evaluated human islet isolation records between January 2001 and February 2003. One hundred forty-two human pancreata were procured from cadaveric donors and preserved by means of the UW solution (n=112) or TLM (n=30). Human islet isolations were performed using a standard protocol and assessed by islet recovery and in vitro function of islets. Results. Eight to ten hours of cold ischemia in the UW solution is a critical point for successful islet isolations. It is difficult to recover a sufficient number of viable islets for transplantation from human pancreata with more than 10 hours of cold storage in the UW solution. The overall islet recovery in the TLM group was significantly higher than in the UW group. With 10 to 16 hours of cold storage, the success rates of islet isolations remained at 62% in the TLM group but decreased to 22% in the UW group. Transplanted islets in the TLM group worked well in the recipients. Conclusions. There are time limitations for using the UW solution for pancreas preservation before human islet isolation. The TLM is a potential method to prolong the optimal cold storage time for successful islet isolations.