The construction and application of a new PPY-MSPQC for l-asparaginase activity assay
The construction and application of a new PPY-MSPQC for l-asparaginase activity assay
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DOI：
10.1016/j.snb.2009.12.006
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发表时间：
2010-03
影响因子：
8.4
通讯作者：
Jiali Ren;F. He;Lingling Zhang
中科院分区：
文献类型：
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作者：
Jiali Ren;F. He;Lingling Zhang
存在对用于NH 3检测的简单且真实的时间传感器的感知需求，其将在室温和高湿度下起作用以与生物应用兼容。为此，提出了一种新的聚吡咯多通道串联压电石英晶体系统（PPY-MSPQC）。在该系统中，聚吡咯化学沉积在聚酰亚胺表面作为探针，而不是平行电极。在室温和高湿条件下，探针都能与NH3发生反应，引起电阻抗的增加，从而引起频率偏移的变化。与平行电极相比，该传感器具有以下优点：（1）传感器能对气体变化作出响应，不受MSPQC传感器灵敏范围的限制;（2）用聚酰亚胺代替金属电极，消除了金属在液体介质中腐蚀的影响。详细讨论了探针制备的最佳条件。L-天冬酰胺酶可将L-天冬酰胺转化为L-天冬氨酸和氨，并诱导急性淋巴细胞白血病（ALL）缓解。选择它作为模型酶，使用所提出的系统检测其活性。在0.01- 0.10 μ ml-1范围内，起始反应速率与酶活力呈线性关系。检测限为0.01Uml−1。与纳氏比色法相比，该方法具有操作简单、无毒、真实的实时性好等优点。
There is a perceived need for a simple and real time sensor for NH3detection which will function at both room temperature and high humidity to be compatible with biological applications. A new polypyrrole multi-channel series piezoelectric quartz crystal system (PPY-MSPQC) is proposed for this purpose. In this system, polypyrrole chemically deposited on the surface of polyimide was used as a probe instead of parallel electrodes. The probe can react with NH3, which causes an increase of electric impedance and results in frequency shift changes at both room temperature and high humidity. Compared with parallel electrode, the new probe has some advantages as follows: (1) the sensor can respond to a gas change and was not limited by the sensitive range of MSPQC; (2) using polyimide instead of metal electrode, the effect of metal corrosion in liquid medium was eliminated. The optimum conditions of probe prepared were discussed in detail. l-Asparaginase can convert l-asparagine to l-aspartic acid and ammonia and induce remission in acute lymphoblastic leukemia (ALL). It was chosen as a model enzyme, the activity of which was detected using proposed system. The initial reaction rate had a linear relationship with the activity of l-asparaginase in the range of 0.01–0.10Uml−1. The detection limit was 0.01Uml−1. Compared with Nesslerization method, proposed method was simple, non-toxic and real time.