System analysis of Phycomyces light-growth response: madC, madG, and madH mutants.
System analysis of Phycomyces light-growth response: madC, madG, and madH mutants.
复制标题
须霉菌光生长反应的系统分析：madC、madG 和 madH 突变体。
DOI：
10.1016/s0006-3495(89)82845-0
复制
发表时间：
1989
影响因子：
3.4
通讯作者：
Lipson,ED
中科院分区：
文献类型：
--
作者：
Palit,A;Pratap,PR;Lipson,ED
在非线性系统辨识的维纳理论框架下，用正弦和法进一步研究了藻类的光生长响应。以光强的对数作为输入，以延伸率作为输出，将响应处理为黑箱。光生长响应的非线性输入-输出关系可以用一组称为核的加权函数在数学上表示，它们出现在维纳积分级数中。用高斯白噪声测试刺激物测定野生型、MADA至MADG基因的单突变体和双突变体的线性(一阶)核，并用来研究这些基因(R.C.Poe、P.pratap和E.D.Lipson)的产物之间的相互作用。1986年。比奥尔。赛伯恩。55：105。)。我们使用了更精确的正弦和方法来扩展相互作用的研究，包括一阶和二阶核。具体地说，我们研究了MadH基因产物与MadC基因产物和MadG基因产物的相互作用。实验是在Phycomyces跟踪机上进行的。刺激的对数平均强度为6×10~(-2)Wm~(-2)，波长为477 nm。用非线性动力学模型对一阶和二阶核进行了分析。
The light-growth response of Phycomyces has been studied further with the sum-of-sinusoids method in the framework of the Wiener theory of nonlinear system identification. The response was treated as a black box with the logarithm of light intensity as the input and elongation rate as the output. The nonlinear input-output relation of the light-growth response can be represented mathematically by a set of weighting functions called kernels, which appear in the Wiener intergral series. The linear (first-order) kernels of wild type, and of single and double mutants affected in genes madA to madG were determined previously with Gaussian white noise test stimuli, and were used to investigate the interactions among the products of these genes (R.C. Poe, P. Pratap, and E.D. Lipson. 1986. Biol. Cybern. 55:105.). We have used the more precise sum-of-sinusoids method to extend the interaction studies, including both the first- and second-order kernels. Specifically, we have investigated interactions of the madH ("hypertropic") gene product with the madC ("night blind") and madG ("stiff") gene products. Experiments were performed on the Phycomyces tracking machine. The log-mean intensity of the stimulus was 6 x 10(-2) W m-2 and the wavelength was 477 nm. The first- and second-order kernels were analyzed in terms of nonlinear kinetic models.(ABSTRACT TRUNCATED AT 250 WORDS)