RESPONSE OF CELL WALLS OF ESCHERICHIA COLI TO A SUDDEN REDUCTION OF ENVIRONMENTAL OSMOTIC PRESSURE
RESPONSE OF CELL WALLS OF ESCHERICHIA COLI TO A SUDDEN REDUCTION OF ENVIRONMENTAL OSMOTIC PRESSURE
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DOI：
10.1128/jb.93.3.1104-1112.1967
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发表时间：
1967-01-01
影响因子：
3.2
通讯作者：
BAYER, ME
中科院分区：
文献类型：
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作者：
BAYER, ME
发现渗透休克后的存活率取决于生长状态。当对数生长时，大肠杆菌对环境渗透压突然下降的敏感度比稳定期高约 20 至 100 倍。对快速生长的细胞进行这种处理会导致细菌壁上出现手指状的突起。我们的结果表明，在这些挤压件下面，壁的刚性层包含薄弱区域，当在电子显微镜下观察时，这些薄弱区域表现为间隙的不连续性。暴露于渗透休克后，间隙变得更大。我们得出的结论是，这些间隙代表了粘聚合物合成的位点，其中刚性结构已被水解酶暂时打开，以允许新的壁材料插入到壁的旧部分中。
The rate of survival after osmotic shocks was found to be dependent on the state of growth. When growing logarithmically, E. coli was about 20 to 100 times more sensitive to an abrupt decrease of the environmental osmotic pressure than when it was in the stationary phase. Subjecting rapidly growing cells to such a treatment caused fingerlike extrusions to emerge from the bacterial wall. Our results suggest that underneath these extrusions the rigid layer of the wall contains weak areas which appear as discontinuities of gaps when viewed in an electron microscope. After exposure to osmotic shock, the gaps became wider. We concluded that the gaps represent sites of mucopolymer synthesis where the rigid structure has temporarily been opened by hydroytic enzymes to allow for the insertion of new wall material into the older portions of the wall.