Polymer/enzyme-modified HF-etched carbon nanoelectrodes for single-cell analysis
Polymer/enzyme-modified HF-etched carbon nanoelectrodes for single-cell analysis
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DOI：
10.1016/j.bioelechem.2020.107487
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发表时间：
2020-06-01
影响因子：
5
通讯作者：
Schuhmann, Wolfgang
中科院分区：
文献类型：
--
作者：
Marquitan, Miriam;Ruff, Adrian;Schuhmann, Wolfgang
碳基纳米电极通过热解的烷烃前体气体吹扫通过玻璃毛细管，随后用HF蚀刻制成的修饰与氧化还原聚合物/酶膜的葡萄糖检测在单细胞水平。葡萄糖氧化酶（GOx）的固定化和电气布线的装置通过Os-配合物-改性的氧化还原聚合物在顺序浸涂过程中。对于氧化还原聚合物基质的合成，使用基于聚（1-乙烯基咪唑-共-丙烯酰胺）的主链，其首先用电子转移介体[Os（bpy）（2）Cl]（+）（bpy = 2，2 '-联吡啶）改性，然后在聚合物类似物反应中将丙烯酰胺单体内的酰胺基团转化为酰肼基团。酰肼基团易于与双官能环氧基交联剂反应，确保高膜稳定性。将纳米尺寸的聚合物/酶修饰电极插入粘附生长的单个NG 108 -15细胞中导致与细胞内葡萄糖浓度相关的正电流响应。此外，纳米传感器显示出稳定的电流输出，在细胞内测量后没有显著的性能损失。(C)2020爱思唯尔B. V.保留所有权利。
Carbon-based nanoelectrodes fabricated by means of pyrolysis of an alkane precursor gas purged through a glass capillary and subsequently etched with HF were modified with redox polymer/enzyme films for the detection of glucose at the single-cell level. Glucose oxidase (GOx) was immobilized and electrically wired by means of an Os-complex-modified redox polymer in a sequential dip coating process. For the synthesis of the redox polymer matrix, a poly(1-vinylimidazole-co-acrylamide)-based backbone was used that was first modified with the electron transfer mediator [Os(bpy)(2)Cl](+) (bpy = 2,2'-bipyridine) followed by the conversion of the amide groups within the acrylamide monomer into hydrazide groups in a polymer-analogue reaction. The hydrazide groups react readily with bifunctional epoxide-based crosslinkers ensuring high film stability. Insertion of the nanometre-sized polymer/enzyme modified electrodes into adherently growing single NG108-15 cells resulted in a positive current response correlating with the intracellular glucose concentration. Moreover, the nanosensors showed a stable current output without significant loss in performance after intracellular measurements. (C) 2020 Elsevier B.V. All rights reserved.