Immediate-early gene induction and MAP kinase activation during recovery from metabolic inhibition in cultured cardiac myocytes.
Immediate-early gene induction and MAP kinase activation during recovery from metabolic inhibition in cultured cardiac myocytes.
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培养的心肌细胞从代谢抑制恢复期间的立即早期基因诱导和 MAP 激酶激活。
DOI：
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发表时间：
1995
影响因子：
15.9
通讯作者：
T. Serizawa
中科院分区：
文献类型：
--
作者：
A. Yao;T. Takahashi;T. Aoyagi;K. Kinugawa;O. Kohmoto;S. Sugiura;T. Serizawa
为了研究心肌细胞如何从短暂的缺血中恢复，我们使用了鸡胚心室肌细胞的代谢抑制（MI）模型（体外缺血模型之一），并检查了立即早期（IE）基因mRNA的诱导和丝裂原激活蛋白（MAP）激酶的活性。我们使用 1 mM NaCN 和 20 mM 2-脱氧-d-葡萄糖进行 Northern 印迹分析，研究 MI 期间以及 MI 30 分钟恢复期间 c-jun、c-fos 和 c-myc mRNA 的表达。在恢复过程中，c-fos mRNA 在 30 分钟和 60 分钟时被瞬时诱导。 c-jun mRNA 的表达在恢复期间在 30、60、90 和 120 分钟显着增强（分别诱导 3.0、4.7、2.4 和 1.9 倍），c-myc mRNA 表达也显着增强（分别诱导 1.4、1.7、1.8 和 2.0 倍）。相反，这些 mRNA 的水平在 MI 期间保持不变。电泳迁移率变动分析显示，在恢复过程中，AP-1 DNA 结合活性在 120 分钟时显着增加。当用蛋白激酶 C (PKC) 抑制剂、100 µM H-7 或 1 µM 星形孢菌素预处理细胞时，恢复期间 60 分钟 c-jun mRNA 的诱导被显着抑制（分别减少 95% 或 82%）。当在 MI 和恢复期间用 2 mM EGTA 处理细胞时，c-jun 诱导受到部分抑制（减少 42%）。通过凝胶内激酶测定定量的 MAP 激酶活性在 MI 期间没有变化，但在恢复期间在 5、10 和 15 分钟时显着增加（分别增加 3.0、4.1 和 3.4 倍）。在恢复过程中 15 分钟，S6 激酶活性也显着增强。因此，这些数据表明IE基因和MAP激酶可能在MI心肌细胞的恢复过程中发挥作用，并且c-jun表达的增强需要PKC的激活，并且在某种程度上需要[Ca2+]i的激活。
To investigate how cardiac myocytes recover from a brief period of ischemia, we used a metabolic inhibition (MI) model, one of the in vitro ischemic models, of chick embryo ventricular myocytes, and examined the induction of immediate-early (IE) genes mRNAs and the activity of mitogen-activated protein (MAP) kinase. We performed Northern blot analysis to study the expression of c-jun, c-fos, and c-myc mRNAs during MI using 1 mM NaCN and 20 mM 2-deoxy-d-glucose, and also during the recovery from MI of 30 min. The c-fos mRNA was induced transiently at 30 and 60 min during the recovery. The expression of c-jun mRNA was significantly augmented at 30, 60, 90, and 120 min during the recovery (3.0-, 4.7-, 2.4-, and 1.9-fold induction, respectively) and so did the expression of c-myc mRNA (1.4-, 1.7-, 1.8-, and 2.0-fold induction, respectively). In contrast, the levels of these mRNAs remained unchanged during MI. The electrophoretic mobility shift assay revealed that AP-1 DNA binding activity markedly increased at 120 min during the recovery. When the cells were pretreated with protein kinase C (PKC) inhibitors, 100 microM H-7 or 1 microM staurosporine, the induction of c-jun mRNA at 60 min during the recovery was markedly suppressed (95 or 82% reduction, respectively). The c-jun induction was partially inhibited when the cells were treated with 2 mM EGTA during MI and the recovery (42% reduction). MAP kinase activity quantified with in-gel kinase assay was unchanged during MI, but significantly increased at 5, 10, and 15 min during the recovery (3.0-, 4.1-, and 3.4-fold increase, respectively). S6 kinase activity was also augmented significantly at 15 min during the recovery. Thus, these data suggest that IE genes as well as MAP kinase may play roles in the recovery process of cardiac myocytes from MI, and that the augmentation of c-jun expression needs the activation of PKC and to some extent, [Ca2+]i.
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心肌细胞氧化还原或代谢应激对 fos 和 jun 立即早期基因的调节。
影响因子：
20.1
作者：
Webster,KA;Discher,DJ;Bishopric,NH
通讯作者：
Bishopric,NH
DOI：
10.1073/pnas.83.21.8348
发表时间：
1986-11-01
影响因子：
11.1
作者：
STARKSEN, NF;SIMPSON, PC;WILLIAMS, LT
通讯作者：
WILLIAMS, LT
ATP 耗竭导致培养的心室肌细胞中 Na 泵密度可逆性降低。
DOI：
10.1152/ajpheart.1993.264.4.h1208
发表时间：
1993
期刊：
The American journal of physiology
影响因子：
--
作者：
Ikenouchi,H;Zhao,L;McMillan,M;Hammond,EM;Barry,WH
通讯作者：
Barry,WH
影响因子：
15.9
作者：
OUELLETTE, AJ;MALT, RA;BONVENTRE, JV
通讯作者：
BONVENTRE, JV
心肌细胞中 Fos 和 Jun 对骨骼 α-肌动蛋白基因的正向调节。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Bishopric,NH;Jayasena,V;Webster,KA
通讯作者：
Webster,KA