Detection and distribution of rotavirus in municipal sewage treatment plants (STPs) and surface water in Beijing
Detection and distribution of rotavirus in municipal sewage treatment plants (STPs) and surface water in Beijing
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DOI：
10.1080/10934520701795731
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发表时间：
2008-01-01
影响因子：
2.1
通讯作者：
Wang, Zi J.
中科院分区：
文献类型：
--
作者：
He, Xiao Q.;Cheng, Li;Wang, Zi J.
本研究的目的是调查北京市污水处理厂（STP）和地表水样品中轮状病毒的存在和分布。此外，还讨论了三个污水处理厂废水处理工艺的轮状病毒去除效果。 SiO2 用于浓缩环境水样中的轮状病毒颗粒。采用逆转录酶巢式聚合酶链式反应（RT-nested PCR）方法检测轮状病毒。几乎所有从 STP 水体（10/10 进水，100%；10/10 二级出水，100%；9/10 再生出水，90%）和河水（14/14 样品，100%）以及一些湖水（37/45 样品，82.2%）、运河水收集的样品中均检测到轮状病毒。 （7/22个样品，31.8%），以及饮用水源地附近的湿地水域（5/26个样品，19.2%）。结果显示，采样期间，轮状病毒在北京不同类型的水域中广泛分布。污水处理厂的污水处理工艺未能有效消除轮状病毒，这可能导致其在 8 月至 1 月期间传播到地表水。这项研究凸显了从大城市的水样中检测轮状病毒的兴趣，中国每年都会发生许多胃肠炎爆发，因此有必要进一步研究监测饮用水源中的轮状病毒。
The aim of this study was to survey on the presence and distribution of rotavirus in sewage treatment plants (STPs) and surface water samples in Beijing. Also, the rotavirus removal efficacies of wastewater treatment processes in three STPs were discussed. SiO2 was used to concentrate rotavirus particles from environmental water samples. A reverse transcriptase-nested polymerase chain reaction (RT-nested PCR) method was used for detection of rotavirus. Rotavirus could be detected from almost all samples collected from STP waters (10/10 influents, 100%; 10/10 secondary effluents, 100%; 9/10 reclaimed effluents, 90%) and river waters (14/14 samples, 100%), and from some lake waters (37/45 samples, 82.2%), canal waters (7/22 samples, 31.8%), as well as wetland waters near drinking water resource (5/26 samples, 19.2%).Our results showed that rotaviruses were widely distributed in different types of waters in Beijing during sampling period. Sewage treatment processes in STPs were not efficient to eliminate rotavirus, which may lead to its spread to surface waters from August to January. This study highlights the interest to detect rotaviruses from water samples in big cities, where many gastroenteritis outbreaks occur each year in China and the results necessitate the further study on monitoring rotavirus in source drinking water.