Noninvasive neuroelectronic interfacing with synaptically connected snail neurons immobilized on a semiconductor chip
Noninvasive neuroelectronic interfacing with synaptically connected snail neurons immobilized on a semiconductor chip
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DOI：
10.1073/pnas.181348698
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发表时间：
2001-08-28
影响因子：
11.1
通讯作者：
Fromherz, P
中科院分区：
文献类型：
--
作者：
Zeck, G;Fromherz, P
设计了一种半导体芯片和突触连接神经元的混合电路，利用聚酰亚胺的微型栅栏将钉螺的单个神经细胞固定在硅芯片上。这些细胞在脑条件培养液中生长后形成带有电突触的网络。对神经元进行电子接口，以进行非侵入性刺激和记录。电压脉冲被施加到芯片上的电容刺激器，以兴奋连接的神经元。信号在神经元网络中传递，并在第二个神经元中引发动作电位。突触后的兴奋调节了芯片上晶体管的电流。硅-神经元-神经元-硅电路的实现构成了神经电子系统开发的原理验证实验，该系统将用于神经元信号处理、神经计算和神经假体的研究。
A hybrid circuit of a semiconductor chip and synaptically connected neurons was implemented and characterized, Individual nerve cells from the snail Lymnaea stagnalis were immobilized on a silicon chip by microscopic picket fences of polyimide. The cells formed a network with electrical synapses after outgrowth in brain conditioned medium. Pairs of neurons were electronically interfaced for noninvasive stimulation and recording. Voltage pulses were applied to a capacitive stimulator on the chip to excite the attached neuron. Signals were transmitted in the neuronal net and elicited an action potential in a second neuron. The postsynaptic excitation modulated the current of a transistor on the chip. The implementation of the silicon-neuron-neuron-silicon circuit constitutes a proof-of-principle experiment for the development of neuroelectronic systems to be used in studies on neuronal signal processing, neurocomputation, and neuroprosthetics.