Role of the Porphyromonas gingivalis iron-binding protein PG1777 in oxidative stress resistance.
Role of the Porphyromonas gingivalis iron-binding protein PG1777 in oxidative stress resistance.
复制标题
牙龈卟啉单胞菌铁结合蛋白 PG1777 在氧化应激抵抗中的作用。
DOI：
10.1099/mic.0.000213
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Fletcher,HanselM
中科院分区：
文献类型：
--
作者：
McKenzie,RachelleME;Henry,LeroyG;Boutrin,Marie-Claire;Ximinies,Alexia;Fletcher,HanselM
Whole genome sequencing of the response ofPorphyromonas gingivalisW83 to hydrogen peroxide revealed an upregulation of several uncharacterized, novel genes. Under conditions of prolonged oxidative stress inP. gingivalis, increased expression of a unique transcriptional unit carrying thegrpE,dnaJand three other hypothetical genes (PG1777,PG1778andPG1779) was observed. The transcriptional start site of this operon appears to be located 91 bp upstream of the translational start, with a potential − 10 region at − 3 nt and a − 35 region at − 39 nt. IsogenicP. gingivalismutants FLL273 (PG1777 : :ermF-ermAM) and FLL293 (PG1779 : :ermF-ermAM) showed increased sensitivity to and decreased survival after treatment with hydrogen peroxide.P. gingivalisFLL273 showed a fivefold increase in the formation of spontaneous mutants when compared with the parent strain after exposure to hydrogen peroxide. The recombinant PG1777 protein displayed iron-binding properties when incubated with FeSO4and Fe(NH4)2(SO4).6H2O. The rPG1777 protein protected DNA from degradation when exposed to hydrogen peroxide in the presence of iron. Taken together, the data suggest that thegrpE-dnaJ-PG1777-PG1778-PG1779transcriptional unit may play an important role in oxidative stress resistance inP. gingivalisvia its ability to protect against DNA damage.
