Differential gene expression profiling of human umbilical cord blood-derived mesenchymal stem cells by DNA microarray
Differential gene expression profiling of human umbilical cord blood-derived mesenchymal stem cells by DNA microarray
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DOI：
10.1634/stemcells.2004-0304
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发表时间：
2005-04-01
期刊：
影响因子：
5.2
通讯作者：
Kim, H
中科院分区：
文献类型：
--
作者：
Jeong, JA;Hong, SH;Kim, H
间充质干细胞（MSC）保留自我更新和多向分化能力。尽管具有广泛的治疗潜力，但人MSC的许多方面，特别是定义干细胞的分子参数，在很大程度上仍然未知。利用高密度寡核苷酸芯片技术，我们获得了人脐带血单个核细胞（UCB）及其MSC亚群之间的差异基因表达谱。特别令人感兴趣的是47个基因的子集在MSC中优先表达50倍或更高，这可以被视为人MSC的分子基础。该亚群包含许多编码胶原蛋白、其他细胞外基质或相关蛋白、细胞因子或生长因子以及细胞因子相关蛋白的基因，但很少有膜和核蛋白的基因。此外，将该微阵列生成的转录组与已发表的基因表达数据的系列分析进行直接比较表明，UCB衍生的MSC的分子背景或多或少与骨髓衍生的细胞相似。总之，我们的研究结果将提供一个基础的分子机制控制的人骨髓间充质干细胞的核心特性的研究。
Mesenchymal stem cells (MSCs) retain both self-renewal and multilineage differentiation capabilities. Despite wide therapeutic potential, many aspects of human MSCs, particularly the molecular parameters to define the sternness, remain largely unknown. Using high-density oligonucleotide microarrays, we obtained the differential gene expression profile between a fraction of mononuclear cells of human umbilical cord blood (UCB) and its MSC subpopulation. Of particular interest was a subset of 47 genes preferentially expressed at 50-fold or higher in MSCs, which could be regarded as a molecular foundation of human MSCs. This subset contains numerous genes encoding collagens, other extracellular matrix or related proteins, cytokines or growth factors, and cytoskeleton-associated proteins but very few genes for membrane and nuclear proteins. In addition, a direct comparison of this microarray-generated transcriptome with the published serial analysis of gene expression data suggests that a molecular context of UCB-derived MSCs is more or less similar to that of bone marrow-derived cells. Altogether, our results will provide a basis for studies on molecular mechanisms controlling core properties of human MSCs.