Ultrastructural features of the spermatozoon and its differentiation in Brandesia turgida (Brandes, 1888) (Digenea, Microphalloidea, Pleurogenidae)
Ultrastructural features of the spermatozoon and its differentiation in Brandesia turgida (Brandes, 1888) (Digenea, Microphalloidea, Pleurogenidae)
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DOI：
10.1007/s00436-014-3897-6
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发表时间：
2014-07-01
影响因子：
2
通讯作者：
Poddubnaya, Larisa G.
中科院分区：
文献类型：
--
作者：
Brunanska, Magdalena;Brazova, Timea;Poddubnaya, Larisa G.
本文首次用透射电镜观察了红腹侧褶蛙（Pelophylax ridibundus，Pallas，1771）的肠道寄生虫--膨胀布兰德氏虫（Brandesia turgida，Brandes，1888）的精子学特征。精子形成的过程开始于分化区的出现，分化区由皮质微管包围，含有两个中心粒，中心粒与横纹小根和中心粒间体相连。中心粒间体由七个不同的电子致密板，两个较低的电子致密板和四个电子透明区组成。两个鞭毛的正交发展其次是鞭毛旋转和他们的proximodistal融合与中间的细胞质过程。这个过程伴随着一个延伸的两个细胞核和细胞核到中间的细胞质过程。B的成熟精子。肿胀体包含两个长度不等的平行轴丝，具有9+ 1”的Trepaxonematan模式、轴突、核、平行皮质微管、四个电子致密附着区、质膜的外部附着和电子致密糖原颗粒。雄配子的前端包含一个完整的中心粒，第二中心粒的中心元件的一个小组件，和外周皮质微管（多达45）。成熟的精子体的后端表现出紊乱的轴丝的管状成分。本研究提供了第一个资料，精子发生在家庭Pleurogenidae。本文还讨论了复殖吸虫属内精子超微结构特征的变异，特别是微腔菌总科不同科之间的差异。
Spermatological characters of the digenean Brandesia turgida (Brandes, 1888), an intestinal parasite of the frog Pelophylax ridibundus (Pallas, 1771), have been investigated by means of transmission electron microscopy for the first time. The process of the spermatozoon formation begins with the appearance of the differentiation zone bordered by cortical microtubules and containing two centrioles associated with striated rootlets and with an intercentriolar body. The intercentriolar body is made up of seven distinct electron-dense plates, two less electron-dense, and four electron-lucent zones. The orthogonal development of the two flagella is followed by a flagellar rotation and their proximodistal fusion with the median cytoplasmic process. This process is accompanied by an extension of both the mitochondrion and nucleus into the median cytoplasmic process. The mature spermatozoon of B. turgida contains two parallel axonemes of unequal lengths with the 9+"1" trepaxonematan pattern, mitochondrion, nucleus, parallel cortical microtubules, four electron-dense attachment zones, an external ornamentation of the plasma membrane, and electron-dense glycogen granules. The anterior extremity of the male gamete contains one complete centriole, a small component of the central element of the second centriole, and peripheral cortical microtubules (up to 45). The posterior extremity of the mature spematozoon exhibits tubular elements of the disorganized axoneme. The present study provides the first data on spermiogenesis within the family Pleurogenidae. Variations of the spermatozoa ultrastructural characters within Digenea, in particular, between different families of the superfamily Microphalloidea, are discussed.