Over-Expression of ERF38 Gene Enhances Salt and Osmotic Tolerance in Transgenic Poplar
Over-Expression of ERF38 Gene Enhances Salt and Osmotic Tolerance in Transgenic Poplar
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ERF38基因的过度表达增强转基因杨的盐和渗透耐受性
DOI：
10.3389/fpls.2019.01375
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发表时间：
2019-11-04
影响因子：
5.6
通讯作者：
Jiang, Tingbo
中科院分区：
文献类型：
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作者：
Cheng, Zihan;Zhang, Xuemei;Jiang, Tingbo
乙烯响应因子（ERF）基因家族在植物的非生物胁迫响应中起着重要作用。本研究从84 K白杨（Populus alba × Populus glandulosa）中克隆了一个盐诱导型ERF基因ERF 38（Potri.006G138900.1），并对其耐盐和耐渗透胁迫功能进行了研究。ERF 38蛋白定位于细胞核，无自激活作用。酵母单杂交结果表明，ERF 38蛋白能与脱水反应元件（DRE）特异性结合。我们成功地将ERF 38基因转入84 K白杨。在盐胁迫和PEG-6000胁迫下，转基因植株的过氧化物酶（POD）和超氧化物歧化酶（SOD）活性、可溶性蛋白含量和脯氨酸含量均显著高于野生型。对于另外两个参数，过氧化氢（H2 O2）和丙二醛（MDA）含量，在胁迫下，与水对照相比，它们在转基因株系中的增量显著低于野生型。此外，在胁迫下，转基因株系中活性氧（ROS）被清除，而野生型（WT）中没有。有趣的是，在逆境胁迫下，与POD和SOD代谢相关的一些基因的表达水平在转基因杨树中显著增加。总之，形态学、生理生化分析表明，过量表达ERF 38基因可以提高转基因白杨的耐盐性和耐渗透性。
Ethylene response factor (ERF) gene family plays an important role in abiotic stress responses. In this study, we isolated a salt-inducible ERF gene, ERF38 (Potri.006G138900.1), from the 84K poplar (Populus alba × Populus glandulosa) and investigated its functions in salt and osmotic tolerance. We identified that ERF38 protein was targeted to nucleus and had no self-activation. Results from yeast-one-hybrid indicated that the ERF38 protein can specifically bind to the dehydration responsive element (DRE). We then successfully transferred the ERF38 gene into the 84K poplar. Under respective salt and polyethylene glycol (PEG)-6000 stresses, four of the physiological traits, including peroxidase (POD) and superoxide dismutase (SOD) activities, soluble protein content, and proline content, increased significantly in the transgenic plants, compared to the wild type. Regarding the other two parameters, hydrogen peroxide (H2O2) and malondialdehyde (MDA) content, their increments in the transgenic lines under the stresses, which were compared to the water control, were significantly low than that of the wild type. In addition, reactive oxygen species (ROS) are scavenged in the transgenic lines under the stresses, but not in the wild type (WT). Interestingly, when challenged with the stresses, expression levels of a few genes associated with POD and SOD metabolism were significantly increased in the transgenic poplars. In all, evidence from morphological, physiological, and biochemical analyses indicated that over-expression of ERF38 gene can improve salt and osmotic tolerance in the transgenic poplar.