Serotonergic neurons migrate radially through the neuroepithelium by dynamin-mediated somal translocation.
Serotonergic neurons migrate radially through the neuroepithelium by dynamin-mediated somal translocation.
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DOI：
10.1523/jneurosci.2333-09.2010
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发表时间：
2010-01-13
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影响因子：
--
通讯作者：
Silver J
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作者：
Hawthorne AL;Wylie CJ;Landmesser LT;Deneris ES;Silver J
胚胎中枢神经系统神经元可以通过放射状胶质细胞引导的运动从脑室区迁移到它们的最终目的地。另一个不太被重视的机制是染色体易位，其中年轻的神经元保持其原始的心室和软膜突起，细胞体通过这些突起移动。研究易位的一个主要问题是识别原始放射状细胞中出现的神经元特异性标记。我们使用增强的黄色荧光蛋白的控制下的宠物-1增强子/启动子区域（ePet-EYFP），一个特定的标记物的早期分化的多巴胺能神经元，研究他们的迁移通过免疫组织学和时间推移成像的活切片培养。早在E10.0，表达ePet-EYFP的神经元是无轴突的，径向取向，并跨越整个神经上皮。索马在软膜突内向软膜表面移位，也可通过从软膜突发出的轴突移位。动力蛋白抑制剂dynasore显着降低易位速度，而非肌肉肌球蛋白II抑制剂blebbistatin和驱动蛋白抑制剂AMP-PNP没有显着影响。在这里，我们第一次表明，多巴胺能神经元迁移介导的染色体易位，部分，发动蛋白。
Embryonic CNS neurons can migrate from the ventricular zone to their final destination by radial glial-guided locomotion. Another less appreciated mechanism is somal translocation, where the young neuron maintains its primitive ventricular and pial processes, through which the cell body moves. A major problem in studying translocation has been the identification of neuronal-specific markers that appear in primitive, radially shaped cells. We used enhanced yellow fluorescent protein under control of the Pet-1 enhancer/promoter region (ePet-EYFP), a specific marker of early differentiated serotonergic neurons, to study their migration via immunohistology and time-lapse imaging of living slice cultures. As early as E10.0, ePet-EYFP-expressing neurons were axonless, radially oriented, and spanned the entire neuroepithelium. The soma translocated within the pial process toward the pial surface and could also translocate through its neurites, which sprouted from the pial process. The dynamin inhibitor dynasore significantly reduced translocation velocity, while the nonmuscle myosin II inhibitor blebbistatin and the kinesin inhibitor AMP-PNP had no significant effect. Here we show for the first time that serotonergic neurons migrate by somal translocation mediated, in part, by dynamin.