Pyruvate dehydrogenase activity in the normal and cryptorchid rat testis.
Pyruvate dehydrogenase activity in the normal and cryptorchid rat testis.
复制标题
正常和隐睾大鼠睾丸中的丙酮酸脱氢酶活性。
作者：
M. J. Free;F. Payvar
本研究测定了正常大鼠睾丸组织匀浆中丙酮酸脱氢酶(PDH)的活性和总水平，以及假手术和隐睾症大鼠睾丸组织匀浆中PDH活性。结果表明，在大鼠睾丸匀浆中确实发生了PDH的失活-活性相互转化，7 mS镁(CID：1)足以最大限度地激活无活性PDH，约40%的PDH为活性PDH。与空白对照组和假手术对照组相比，睾丸移位4h后PDH活性增加了50%(P<；0.001)。8h时，PDH活性较对照组低30%(P<；0.01)。长期易位导致PDH活性在第14、16和120天分别减少了70%、60%和60%。
Studies were carried out to determine the active and total levels of pyruvate dehydrogenase (PDH) in normal rat testis tissue homogenates and PDH activity in 2-, 4-, and 8-h and 14-, 16-, and 120-day sham-operated and cryptorchid rat testis homogenates. It was confirmed that the inactive-active interconversion of PDH does take place in rat testicular homogenates, that 7 mas Mg(cid:1) was sufficient for maximal activation of the inactive form, and that approximately 40% of total PDH was in the active form. PDH activity increased by 50% 4 h after al(cid:1)dominal translocation of the testes compared with zero time or sham-operated controls (P <0.001). By 8 h, PDH activity was 30% lower than controls (P <0.01). Longer term translocation resulted in 70, 60, and 60% reductions in PDH activity at 14, 16, and 120 days, respectively.