Inhibition of taurine and 5'AMP olfactory receptor sites of the spiny lobster Panulirus argus by odorant compounds and mixtures.
Inhibition of taurine and 5'AMP olfactory receptor sites of the spiny lobster Panulirus argus by odorant compounds and mixtures.
复制标题
气味化合物和混合物对大龙虾 Panulirus argus 的牛磺酸和 5AMP 嗅觉受体位点的抑制。
DOI：
10.1007/bf00196418
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Derby,CD
中科院分区：
文献类型：
--
作者：
Olson,KS;Derby,CD
1.研究了5′-磷酸腺苷（5′AMP）、铵盐、甜菜碱、L-半胱氨酸、L-谷氨酸、DL-琥珀酸、和牛磺酸及其混合物对牛磺酸和5′AMP与刺龙虾嗅器树突膜结合的影响（Panulirus argus）进行定量，以评估气味抑制的贡献，2.牛磺酸结合位点属于两类亲和性位点，而5′AMP结合位点属于单一亲和性位点。牛磺酸或5′AMP的结合被大多数气味化合物以明显的非竞争性、浓度依赖性方式部分抑制，1 mM气味化合物的抑制率为25- 40%。两种或多种气味化合物的混合物也抑制牛磺酸或5′AMP与其位点的结合。然而，混合物的抑制作用往往显着低于预期的抑制作用所产生的混合物的成分假设无论是非竞争性或竞争机制。3.通过将这种化合物之间的结合抑制作用纳入模型预测的生理反应的混合物从响应的组件，模型的预测能力显着提高。这一结果强烈表明，结合抑制可以影响化学感受器细胞的生理反应的混合物。
1.The effects of the odorant compounds adenosine-5′-monophosphate (5′AMP), ammonium, betaine, L-cysteine, L-glutamate, DL-succinate, and taurine and of mixtures of these comounds on binding of taurine and 5′AMP to dendritic membrane from the olfactory organ of spiny lobsters (Panulirus argus) were quantified to evaluate the contribution of inhibition of odorant-receptor binding to the generation of physiological responses to mixtures.2.Taurine binding sites belong to two affinity classes, while 5′AMP binding sites belong to a single affinity class. Binding of either taurine or 5′AMP was partially inhibited in an apparently noncompetitive, concentration dependent fashion by most odorant compounds, with 25–40 % inhibition by 1 mMof odorant. Mixtures of two or more odorant compounds also inhibited binding of taurine or 5′AMP to its sites. However, the inhibition by mixtures was often significantly less than expected from the inhibition produced by a mixture's components assuming either a noncompetitive or competitive mechanism.3.By including this binding inhibition between compounds into models for predicting physiological responses to mixtures from the responses to the components, the predictive power of the models is significantly improved. This result strongly suggests that binding inhibition can influence the physiological responsiveness of chemoreceptor cells to mixtures.