Features of the replicon of plasmid pAM10.6 of Pseudomonas fluorescens.
Features of the replicon of plasmid pAM10.6 of Pseudomonas fluorescens.
复制标题
荧光假单胞菌质粒pAM10.6复制子的特征。
作者：
M. Peters;E. Jõgi;I. Suitso;T. Punnisk;A. Nurk
我们描述了10.6kb中拷贝数质粒pAM10.6的基本复制子的特征。PAM10.6能够在各种假单胞菌中复制，但只有在插入P15A复制起点后才能在大肠杆菌中保持。缺失分析表明，pAM10.6的复制起点位于一个0.5kb的区域，其中包括REPA基因上游的反向重复和正向重复。REPA(204氨基酸)与其他一些革兰氏阴性细菌的质粒复制蛋白有明显的同源性。PAS(质粒成瘾系统)(编码在480-bp区域的基因)在非选择性条件下稳定恶臭假单胞菌细胞中的质粒维持至少200代。PAM10.6的一个3.75kb的PstI片段与Km(R)基因相连，是恶臭假单胞菌中以单体形式存在的最小质粒单位。该3.75kb片段的进一步缺失导致恶臭假单胞菌形成稳定的头尾二聚体。
We describe features of the basic replicon of the 10.6-kb medium-copy-number plasmid pAM10.6. pAM10.6 was able to replicate in various Pseudomonas strains but was maintained in Escherichia coli only after the p15A origin of replication was inserted. Deletion analysis suggests that the pAM10.6 origin of replication is located in a 0.5-kb region that includes inverted and direct repeats upstream of the repA gene. RepA (204 aa) has a clear homology to plasmid replication proteins of some other gram-negative bacteria. The pas (plasmid addiction system) (genes encoded in the region of 480-bp) stabilizes plasmid maintenance in P. putida cells under nonselective conditions for at least 200 generations. A 3.75-kb PstI fragment of pAM10.6 joined to a Km(r) gene was shown to be a minimal plasmid unit maintained in P. putida as a monomer. Further deletions of this 3.75-kb fragment caused a drive to form stable head-to-tail dimeric plasmids in P. putida.
大肠杆菌鞭毛开关蛋白 FliG 的基因序列、过量生产、纯化和野生型水平的测定。
影响因子：
3.5
作者：
Roman,SJ;Frantz,BB;Matsumura,P
通讯作者：
Matsumura,P