Poly(glycerol sebacate) supports the proliferation and phenotypic protein expression of primary baboon vascular cells
Poly(glycerol sebacate) supports the proliferation and phenotypic protein expression of primary baboon vascular cells
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DOI：
10.1002/jbm.a.31434
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发表时间：
2007-12-15
影响因子：
4.9
通讯作者：
Wang, Yadong
中科院分区：
文献类型：
--
作者：
Gao, Jin;Ensley, Ann E.;Wang, Yadong
聚甘油癸二酸酯（PGS）是一种生物可降解和生物相容性弹性体，专门为软组织工程开发。粘在弹性体上的血管细胞可能表现出更多的生理行为，因为基质的机械性能与组织的力学性能更接近。为了探讨PGS作为血管组织工程支架材料的可行性，作者研究了在PGS膜和支架上培养的狒狒内皮祖细胞（BaEPCs）和狒狒平滑肌细胞（BaSMCs）的粘附、增殖、表型和形态学特性。用组织培养处理过的聚苯乙烯板作为对照。相差显微镜显示两种类型的细胞在PGS膜上形态正常。免疫荧光染色显示，BaEPCs和BaSMCs分别表达血管性血友病因子和-平滑肌肌动蛋白。两种类型的细胞在PGS表面增殖良好。组织学评价表明，在PGS支架中培养时，basmc分布在整个支架中并合成细胞外基质。扫描电镜证实了basmc在构建体中的分布。共培养构建体的免疫荧光染色表明，以basmc为种子的构建体为BaEPC粘附提供了合适的表面，两种类型的细胞均保持了其特异性表型。这些结果表明PGS是一种合适的血管组织工程支架材料。(c) 2007 Wiley期刊公司
Poly(glycerol sebacate) (PGS) is a biodegradable and biocompatible elastomer specifically developed for soft tissue engineering. Vascular cells adhered to an elastomer may exhibit more physiological behavior because the substrate's mechanical properties more closely match those of the tissue. To investigate the feasibility of using PGS as a scaffold material for vascular tissue engineering, the authors examined the adhesion, proliferation, and phenotypic and morphologic properties of primary baboon endothelial progenitor cells (BaEPCs) and baboon smooth muscle cells (BaSMCs) cultured on PGS films and scaffolds. Tissue culture-treated polystyrene plates were used as controls. Phase contrast microscopy indicated that both types of cells showed normal morphology on PGS films. Immuofluorescent staining revealed that von Willebrand factor and alpha-smooth muscle actin were expressed by BaEPCs and BaSMCs, respectively. Both types of cells proliferated well on PGS surfaces. When cultured in PGS scaffolds, BaSMCs were distributed throughout the scaffolds and synthesized extracellular matrix, as indicated by histological evaluations. The distribution of the BaSMCs in the constructs was confirmed by scanning electron microscopy. Immunofluorescent staining of cocultured constructs indicated that the BaSMC-seeded constructs provided suitable surfaces for BaEPC adhesion, and both types of cells maintained their specific phenotypes. These results suggest that PGS is an appropriate scaffold material for blood vessel tissue engineering. (c) 2007 Wiley Periodicals, Inc.