Predominance of Mother-to-Child Transmission of Helicobacter pylori Infection Detected by Random Amplified Polymorphic DNA Fingerprinting Analysis in Japanese Families
Predominance of Mother-to-Child Transmission of Helicobacter pylori Infection Detected by Random Amplified Polymorphic DNA Fingerprinting Analysis in Japanese Families
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DOI：
10.1097/inf.0b013e31817d756e
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发表时间：
2008-11-01
影响因子：
3.6
通讯作者：
Fujii, Nobuhiro
中科院分区：
文献类型：
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作者：
Konno, Mutsuko;Yokota, Shin-ichi;Fujii, Nobuhiro
背景：幽门螺杆菌（Helicobacter pylori， H. pylori）是人类最常见的致病菌之一，但其传播途径尚不清楚。我们通过DNA指纹分析研究了儿童患者及其家庭成员培养的幽门螺杆菌的传播情况。方法：诊断为幽门螺杆菌胃炎合并或不合并十二指肠/胃溃疡的42例患者，平均年龄11.7岁（范围4 ~ 19岁）。对66名幽门螺杆菌粪便抗原试验和/或血清幽门螺杆菌IgG试验阳性的家庭成员进行了内镜检查、活检或胃液抽吸进行幽门螺杆菌培养。提取幽门螺杆菌基因组DNA并进行pcr - RAPD分析。结果：42例患者中有32例（76%）的DNA指纹模式至少与各自家庭成员中的一名相同。29例（69%）幽门螺杆菌感染患者的分析模式与他们的母亲相同。其中7名患者的模式与他们的父亲相同，其中6名患者的模式也与他们的母亲相同。母亲组与父亲组的指纹图谱相同率显著高于父亲组（P < 0.01）。结论：在日本，母婴传播是幽门螺杆菌感染的主要途径。
Background: Helicobacter pylori (H. pylori) is one of the most common bacterial pathogens in humans but the route of transmission remains unclear. We investigated transmission by DNA fingerprinting analysis of cultured H. pylori from pediatric patients and their family members.Methods: Forty-two index patients with a mean age of 11.7 years (range, 4-19) were diagnosed as having H, pylori gastritis with or without duodenal/gastric ulcer disease. A total of 66 family members for whom the results of the H. pylori stool antigen test and/or serum H. pylori IgG test were positive underwent endoscopic examination and biopsy or aspiration of gastric juice for H. pylori culture. The extraction of H. pylori genomic DNA and PCR-based RAPD analysis were performed.Results: Thirty-two (76%) of the 42 patients showed DNA fingerprint patterns identical to those of at least one of the respective family members. The patterns of 29 (69%) of the analyses of the H. pylori infected patients were identical to those of their mothers. The patterns for 7 patients were identical to those of their fathers, and those for 6 of the latter patients were also identical to those of their mothers. The rate of fingerprint patterns identical to those of the index patients was significantly higher in those of mothers compared with those of fathers (P < 0.01).Conclusions: Mother-to-child transmission is the predominant route of H. pylori infection in Japan.