Density and migration of mast cells in lip squamous cell carcinoma and actinic cheilitis
Density and migration of mast cells in lip squamous cell carcinoma and actinic cheilitis
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DOI：
10.14670/hh-24.457
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发表时间：
2009-04-01
影响因子：
2
通讯作者：
Batista, Aline Carvalho
中科院分区：
文献类型：
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作者：
Costa, Nadia Lago;Oton-Leite, Angelica Ferreira;Batista, Aline Carvalho
肥大细胞（MC）显示出多种作用，可能有助于肿瘤进展过程中基质微环境的改变。本研究的目的是研究类胰蛋白酶和c-kit在唇鳞状细胞癌（唇SCC）（n=37），光化性唇炎（AC）（n=15）和正常唇粘膜（对照组）（n=6）中的MC群体表达，以及它们与显微镜参数（胶原变性，弹性蛋白变化，血管生成和增殖指数）的关系。免疫组化法检测类胰蛋白酶、c-kit、CD 31和Ki-67的表达，Picrosirus和Verhoeff染色观察胶原纤维和弹性纤维。类胰蛋白酶+MC的数量在唇鳞癌中显著高于对照组（P=0.01），而在AC和唇鳞癌中观察到这些细胞的密度相似（P=0.09）。各组c-kit+ MC密度相似（P=0.65）。MC迁移（c-kit+/类胰蛋白酶+关系）在唇SCC中为69%，在AC中为60%，在对照中为100%。CD 31+血管在唇鳞癌中的数量显著高于对照组和腺癌（P
Mast cells (MCs) display a diversity of roles that may contribute to the stromal microenvironment alterations during tumor progression. The aim of this study was to investigate MC populations expressing tryptase and c-kit in lip squamous cell carcinoma (lip SCC) (n=37), actinic cheilitis (AC) (n=15) and normal lip mucosa (control) (n=6), as well as their relationship with microscopic parameters (collagen degeneration, elastin changes, angiogenesis and proliferative index). Tryptase, c-kit, CD31 and Ki-67 expressions were analyzed by means of immunohistochemistry and collagen and elastic fibers were visualized with Picrosirus and Verhoeff's stain, respectively. The numbers of tryptase+MC were significantly higher in lip SCC when compared with control (P=0.01), while a similar density of these cells was observed in AC and lip SCC (P=0.09). The density of c-kit+ MC was similar in all groups examined (P=0.65). MC migration (c-kit+/Tryptase+ relationship) was 69% in lip SCC, 60% in AC and 100% in control. The number of CD31+ blood vessels was significantly higher in the lip SCC when compared with control and AC (P