STRUCTURE AND FUNCTIONAL EXPRESSION OF A HUMAN INTERLEUKIN-8 RECEPTOR
STRUCTURE AND FUNCTIONAL EXPRESSION OF A HUMAN INTERLEUKIN-8 RECEPTOR
复制标题
DOI：
10.1126/science.1840701
复制
发表时间：
1991-09-13
期刊：
影响因子：
56.9
通讯作者：
WOOD, WI
中科院分区：
文献类型：
--
作者：
HOLMES, WE;LEE, J;WOOD, WI
白细胞介素-8（IL-8）是促炎细胞因子家族的成员。虽然IL-8的最佳特征活性包括嗜中性粒细胞的化学吸引和活化，但该家族的其他成员具有广泛的特异性作用，包括单核细胞的趋化性和活化、记忆T细胞的选择性趋化性、造血干细胞增殖的抑制和体内嗜中性粒细胞浸润的诱导。编码来自人嗜中性粒细胞的IL-8受体的互补DNA现已被分离。氨基酸序列表明，该受体是与鸟嘌呤核苷酸结合蛋白（G蛋白）偶联的受体超家族的成员。该序列与其他中性粒细胞化学引诱物fMet-Leu-Phe和C5 a的受体的序列有29%相同。用IL-8受体cDNA克隆转染的哺乳动物细胞以高亲和力结合IL-8，并通过瞬时动员钙对IL-8产生特异性反应。IL-8受体可能是相关G蛋白偶联受体亚家族的一部分，其为促炎细胞因子的IL-8家族传递信号。
Interleukin-8 (IL-8) is a member of a family of pro-inflammatory cytokines. Although the best characterized activities of IL-8 include the chemoattraction and activation of neutrophils, other members of this family have a wide range of specific actions including the chemotaxis and activation of monocytes, the selective chemotaxis of memory T cells, the inhibition of hematopoietic stem cell proliferation, and the induction of neutrophil infiltration in vivo. A complementary DNA encoding the IL-8 receptor from human neutrophils has now been isolated. The amino acid sequence shows that the receptor is a member of the superfamily of receptors that couple to guanine nucleotide binding proteins (G proteins). The sequence is 29% identical to that of receptors for the other neutrophil chemoattractants, fMet-Leu-Phe and C5a. Mammalian cells transfected with the IL-8 receptor cDNA clone bind IL-8 with high affinity and respond specifically to IL-8 by transiently mobilizing calcium. The IL-8 receptor may be part of a subfamily of related G protein-coupled receptors that transduce signals for the IL-8 family of pro-inflammatory cytokines.