DISTRIBUTION OF 2 URINARY RIBONUCLEASE-LIKE ENZYMES IN HUMAN ORGANS AND BODY-FLUIDS
DISTRIBUTION OF 2 URINARY RIBONUCLEASE-LIKE ENZYMES IN HUMAN ORGANS AND BODY-FLUIDS
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DOI：
10.1093/oxfordjournals.jbchem.a135456
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发表时间：
1986-01-01
影响因子：
2.7
通讯作者：
IRIE, M
中科院分区：
文献类型：
--
作者：
MORITA, T;NIWATA, Y;IRIE, M
为了确定两种人尿RNase样酶(RNase US和RNase UL)在人体组织和体液中的分布，用兔抗RNase血清建立了酶免疫分析体系。这些检测系统的灵敏度与先前报道的放射免疫检测系统的灵敏度相似。在酶免疫分析中，抗RNase UL血清与RNase US、牛肾RNase K2、牛RNase A和牛精液RNase VS的交叉反应率均小于1%。RNase抗血清与RNase UL的交叉反应率小于0.5%，与RNase A、RNase K2和RNase VS的交叉反应最小。用酶免疫法测定人体器官和体液中的核糖核酸酶水平。在牛奶、精液和唾液中仅发现核糖核酸酶UL-酶。肾脏、胃和胰腺均有RNase US和RNase UL酶，RNase US/RNase UL比值分别为0.49、1.35和0.34。在肺、肝、脾和白细胞中，大部分核糖核酸酶活性是由类核糖核酸酶决定的。类核糖核酸酶在肺、脾和白细胞中的活性尤其高。用磷酸纤维素柱层析分离几种组织和体液的粗提物，用酶免疫法测定尿液中两种核糖核酸酶的含量。在胃、肾、胰腺和血清中，主要核糖核酸酶(RNase Us)和次要核糖核酸酶(RNase UL)均以两种形式存在。肝脏和白细胞的核糖核酸酶均呈单峰分布。肝、白细胞、脾和肺的核糖核酸酶活性染色表明，核糖核酸酶在这些组织中也以多种形式存在。
In order to determine the distribution of two human urinary RNase (RNase Us and RNase UL)-like enzymes in human tissues and body fluids, enzyme immunoassay systems were established using rabbit anti-RNase sera. The sensitivity of the assay systems was of similar order to that of radioimmunoassay systems previously reported. In the enzyme immunoassay, the cross reactivities of anti-RNase UL serum towards RNase Us, bovine kidney RNase K2, bovine RNase A, and bovine seminal RNase VS were less than 1%. The cross reactivity of anti-RNase Us-serum towards RNase UL was less than 0.5% and cross reactivities were minimal for RNase A, RNase K2, and RNase VS. The RNase levels in human organs and body fluids were measured by enzyme immunoassay. In milk, semen and saliva, only RNase UL-enzyme was found. Both RNase Us- and RNase UL-enzymes were found in kidney, stomach, and pancreas and the RNase Us/RNase UL ratios were 0.49, 1.35, and 0.34, respectively. In lung, liver, spleen, and leukocytes, most of the RNase activity was accounted for by RNase Us-like enzyme. The activity of RNase Us-like enzyme was especially high in lung, spleen, and leukocytes. The crude extracts of several tissues and body fluids were separated by phosphocellulose column chromatography and the contents of the two urinary RNase-like enzymes were determined by enzyme immunoassay. In stomach, kidney, pancreas, and serum, both the major RNase (RNase Us) and minor RNase (RNase UL) existed in two forms. A single peak of RNase was found in liver and in leukocytes. The activity staining of liver, leukocytes, spleen, and lung RNases indicated that RNase Us-like enzyme in these tissues is also present in multiple forms.