Quantitative evaluation of fundus autofluorescence imaged "in vivo" in eyes with retinal disease
Quantitative evaluation of fundus autofluorescence imaged "in vivo" in eyes with retinal disease
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DOI：
10.1136/bjo.84.7.741
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发表时间：
2000-07-01
影响因子：
4.1
通讯作者：
Fitzke, FW
中科院分区：
文献类型：
--
作者：
Lois, N;Halfyard, AS;Fitzke, FW
目的：探讨一种新的眼底自发荧光分布的定量分析方法。方法：应用激光共聚焦扫描检眼镜（cSLO）对5例视网膜病变患者和34例正常人进行眼底自发荧光图像分析。为了评价眼底自发荧光在整个后极的地形分布，使用10 x 750像素的矩形框作为分析区域。盒子水平放置在黄斑区。将矩形框内每个像素的眼底自发荧光的强度相对于其偏心度作图。将患者的眼底自发荧光轮廓与年龄匹配的对照组和cSLO图像中获得的轮廓进行比较。结果-眼底自发荧光轮廓似乎代表了cSLO图像中整个后极的眼底自发荧光的地形分布，并允许快速识别和定量增加或减少眼底自发荧光的区域。眼底自发荧光分布似乎是有用的，以研究眼底自发荧光的空间分布在眼睛与视网膜疾病。
Aim-To describe a new method of evaluating the topographic distribution of fundus autofluorescence in eyes with retinal disease.Methods-Images of fundus autofluorescence were obtained in five patients and 34 normal volunteers using a confocal scanning laser ophthalmoscope (cSLO). To evaluate the topographic distribution of fundus autofluorescence throughout the posterior pole a rectangular box, 10 x 750 pixels, was used as the area of analysis. The box was placed, horizontally, across the macular region. The intensity of fundus autofluorescence of each pixel within the rectangular box was plotted against its degree of eccentricity. Profiles of fundus autofluorescence from patients were compared with those obtained from the age matched control group and with cSLO images.Results-Profiles of fundus autofluorescence appeared to represent the topographic distribution of fundus autofluorescence throughout the posterior pole appreciated in the cSLO images, and allowed rapid identification and quantification of areas of increased or decreased fundus autofluorescence.Conclusions-Fundus autofluorescence profiles appear to be useful to study the spatial distribution of fundus autofluorescence in eyes with retinal disease.