Comparative pathogenicity of different subtypes of duck hepatitis A virus in Pekin ducklings
Comparative pathogenicity of different subtypes of duck hepatitis A virus in Pekin ducklings
复制标题
鸭甲型肝炎病毒不同亚型对北京小鸭致病力的比较
DOI：
10.1016/j.vetmic.2018.11.030
复制
发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Fu, Qiuling
中科院分区：
文献类型：
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作者：
Liu, Rongchang;Shi, Shaohua;Fu, Qiuling
鸭甲型肝炎病毒（DHAV）是鸭病毒性肝炎的主要病原，是雏鸭的一种致死性接触传染病。尽管鉴定了许多DHAV毒株（例如DHAV-3、DHAV-2、DHAV-1和DHAV-1a），但尚未评价不同亚型之间的致病性差异。本研究的目的是比较中国大陆流行株DHAV-3、DHAV-1和DHAV-1a在北京鸭感染模型中的致病特性。我们评估了这些不同亚型的致病性调查的临床症状，宏观和微观病变，免疫组织化学检查，和病毒RNA检测后，实验接种北京雏鸭与三个不同的DHAV株。DHAV-3和DHAV-1的致病性差异不显著。DHAV-1a的致病性与经典的鸭甲型肝炎（DHAV-3或DHAV-1）的致病性显著不同，因为没有角弓反张的临床症状。更重要的是，胰腺出血或变黄，脾脏肿胀和出血是DHAV-1a组的主要病变，而肝脏和脾脏病变是经典肝炎（DHAV-1/3）的主要体征。本研究结果表明，鸭肝炎病毒不同亚型对雏鸭的致病性存在差异，这可能有助于了解鸭肝炎病毒不同亚型的生物学特性。
Duck hepatitis A virus (DHAV) is a major pathogen of viral hepatitis in ducks, which is a fatal and contagious disease of young ducklings. Despite the identification of numerous DHAV strains (e.g. DHAV-3, DHAV-2, DHAV-1 and DHAV-1a), the pathogenic differences among the different subtypes have not been evaluated. The objective of this study was to compare the pathogenic properties of three epidemic strains DHAV-3, DHAV-1, and DHAV-1a in mainland China, in a Pekin duckling infection model. We evaluated the pathogenicity of these different subtypes by investigating clinical signs, macroscopic and microscopic lesions, immunohistochemical examination, and viral RNA detection after experimental inoculation of Pekin ducklings with the three different DHAV strains. There was no significant difference in pathogenicity between DHAV-3 and DHAV-1. Pathogenicity of DHAV-1a differed significantly from that of classical duck hepatitis A (DHAV-3 or DHAV-1), in that there were no clinical signs of opisthotonos. More importantly, pancreatic bleeding or yellowing, and spleen swelling and bleeding were the predominant lesions in the DHAV-la group, while liver and spleen lesions were the main signs in classical hepatitis (DHAV-1/3). Our findings indicate that there are differences in the pathogenicity of different subtypes of DHAV in ducklings, which may be useful for understanding the biological characteristics of the different subtypes of DHAV in ducks.