Multinucleated cells in pigmented villonodular synovitis and giant cell tumor of tendon sheath express features of osteoclasts.
Multinucleated cells in pigmented villonodular synovitis and giant cell tumor of tendon sheath express features of osteoclasts.
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发表时间：
1997-04
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通讯作者：
J. Darling;S. Goldring;Y. Harada;M. Handel;J. Glowacki;E. Gravallese
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作者：
J. Darling;S. Goldring;Y. Harada;M. Handel;J. Glowacki;E. Gravallese
色素沉着绒毛结节性滑膜炎（PVNS）和组织学相关病变腱鞘巨细胞瘤（GCTTS）是可诱导骨质溶解和骨囊肿形成的特发性增生性病变。这些病变包含两种主要的细胞类型：单核多面体细胞和多核细胞（MNC）。先前的研究表明，单核细胞表现出与单核细胞/巨噬细胞谱系的衍生一致的表型特征。MNCs的细胞谱系及其与破骨细胞的关系尚不清楚。为了表征这些病变中的MNCs，并建立这些MNCs与破骨细胞的关系，对6例PVNS和2例GCTTS的组织切片进行了研究。单核细胞表达CD 14和HLA-DR，与它们与单核细胞/巨噬细胞谱系的细胞的关系一致。MNCs的表征揭示了与破骨细胞表型相关的特征。8份标本中有7份含有强烈抗酒石酸酸性磷酸酶阳性的MNC;约5%的单核细胞抗酒石酸酸性磷酸酶阳性，这些细胞倾向于包围MNC。两种病变中的MNC与识别α V β 3整联蛋白（玻连蛋白受体）的23 C6单克隆抗体反应强烈，MNC周围的几个单核细胞也是如此。大多数MNCs不表达CD 14或HLA-DR。降钙素受体的表达，破骨细胞的标志物，检测MNCs孵育后的部分与125碘标记的鲑鱼降钙素和乳液放射自显影。6份PVNS样本中的4份和2份GCTTS样本中的2份中的MNC显示特异性降钙素结合。降钙素受体mRNA的表达证实在所有情况下，通过逆转录聚合酶链反应。这些结果表明，PVNS和GCTTS中的MNCs表达真正的破骨细胞的表型特征，并表明破骨细胞样多核细胞可以出现在远离骨的滑膜软组织中。
Pigmented villonodular synovitis (PVNS) and the histologically related lesion giant cell tumor of tendon sheath (GCTTS) are idiopathic, proliferative lesions that can induce osteolysis and formation of bone cysts. These lesions contain two predominant cell types: mononuclear polyhedral cells and multinucleated cells (MNCs). Previous studies demonstrated that the mononuclear cells exhibit phenotypic features consistent with derivation from a monocyte/macrophage lineage. The cell lineage of the MNCs and their relationship to osteoclasts are not known. To characterize the MNCs in these lesions and to establish the relationship of these MNCs to osteoclasts, histological sections from six cases of PVNS and two cases of GCTTS were studied. Mononuclear cells expressed CD14 and HLA-DR, in keeping with their relationship to cells of the monocyte/macrophage lineage. Characterization of the MNCs revealed features associated with an osteoclast phenotype. Seven of the eight specimens contained MNCs that were intensely tartrate-resistant acid phosphatase positive; approximately 5% of the mononuclear cells were tartrate-resistant acid phosphatase positive, and these tended to surround MNCs. MNCs in both lesions reacted strongly with the 23C6 monoclonal antibody that recognizes the alpha V beta 3 integrin (the vitronectin receptor), as did several mononuclear cells surrounding the MNCs. Most MNCs did not express CD14 or HLA-DR. Expression of receptors for calcitonin, a marker for osteoclasts, was detected on MNCs after incubation of sections with 125I-labeled salmon calcitonin and emulsion autoradiography. MNCs in four of six PVNS and two of two GCTTS samples demonstrated specific calcitonin binding. Expression of mRNA for calcitonin receptor was confirmed in all cases by reverse transcriptase polymerase chain reaction. These results demonstrate that MNCs in PVNS and GCTTS express phenotypic features of authentic osteoclasts and suggest that osteoclast-like multinucleated cells can arise in synovial soft tissues remote from bone.