The vaccinia virus based Sementis Copenhagen Vector vaccine against Zika and chikungunya is immunogenic in non-human primates
The vaccinia virus based Sementis Copenhagen Vector vaccine against Zika and chikungunya is immunogenic in non-human primates
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DOI：
10.1038/s41541-020-0191-8
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发表时间：
2020-06-02
期刊：
影响因子：
9.2
通讯作者：
Suhrbier, Andreas
中科院分区：
文献类型：
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作者：
Prow, Natalie A.;Liu, Liang;Suhrbier, Andreas
Sementis Copenhagen Vector（SCV）是一种新的牛痘病毒衍生的、繁殖缺陷的疫苗技术，本文在非人灵长类动物中进行了评估。用编码寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）的结构多蛋白的多病原体重组SCV疫苗接种印度恒河猴（Macaca mulatta）。在一次疫苗接种后，产生了对ZIKV和代表不同病毒基因型的四种CHIKV毒株的中和抗体应答。第二次疫苗接种导致对ZIKV和CHIKV的中和抗体应答的显著加强。在用ZIKV攻击后，与接受对照SCV疫苗的动物相比，SCV-ZIKA/CHIK疫苗接种的动物显示出病毒感染的显著降低。两次SCV疫苗接种也产生了对牛痘病毒的中和和IgG ELISA抗体应答。这些结果证明了重组SCV疫苗在非人灵长类动物中有效诱导免疫力，并说明了SCV作为能够递送多种大免疫原的多疾病疫苗平台的实用性。
The Sementis Copenhagen Vector (SCV) is a new vaccinia virus-derived, multiplication-defective, vaccine technology assessed herein in non-human primates. Indian rhesus macaques (Macaca mulatta) were vaccinated with a multi-pathogen recombinant SCV vaccine encoding the structural polyproteins of both Zika virus (ZIKV) and chikungunya virus (CHIKV). After one vaccination, neutralising antibody responses to ZIKV and four strains of CHIKV, representative of distinct viral genotypes, were generated. A second vaccination resulted in significant boosting of neutralising antibody responses to ZIKV and CHIKV. Following challenge with ZIKV, SCV-ZIKA/CHIK-vaccinated animals showed significant reductions in viremias compared with animals that had received a control SCV vaccine. Two SCV vaccinations also generated neutralising and IgG ELISA antibody responses to vaccinia virus. These results demonstrate effective induction of immunity in non-human primates by a recombinant SCV vaccine and illustrates the utility of SCV as a multi-disease vaccine platform capable of delivering multiple large immunogens.