CHELATING AND FREE-RADICAL SCAVENGING MECHANISMS OF INHIBITORY-ACTION OF RUTIN AND QUERCETIN IN LIPID-PEROXIDATION
CHELATING AND FREE-RADICAL SCAVENGING MECHANISMS OF INHIBITORY-ACTION OF RUTIN AND QUERCETIN IN LIPID-PEROXIDATION
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DOI：
10.1016/0006-2952(89)90410-3
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发表时间：
1989-06-01
影响因子：
5.8
通讯作者：
POTAPOVITCH, AI
中科院分区：
文献类型：
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作者：
AFANASEV, IB;DOROZHKO, AI;POTAPOVITCH, AI
研究了类黄酮芦丁和槲皮素对卵磷脂脂质体亚铁离子依赖性脂质过氧化以及大鼠肝微粒体中 NADPH 和 CCl4 依赖性脂质过氧化的抑制作用，以阐明这些化合物的螯合和自由基清除活性。还研究了芦丁与超氧离子和亚铁离子的相互作用以及槲皮素与脂质过氧自由基的反应。由于螯合铁离子并形成惰性铁复合物而无法引发脂质过氧化，这两种黄酮类化合物都是铁离子依赖性脂质过氧化系统明显更有效的抑制剂。同时，类黄酮的铁络合物保留了其自由基清除活性。芦丁的螯合抑制机制比槲皮素更重要。还研究了芦丁和抗坏血酸对非酶脂质过氧化的相互影响。结论是芦丁和槲皮素能够在三个阶段抑制自由基过程：超氧离子的形成、芬顿反应中羟基（或隐羟基）自由基的生成以及脂质过氧自由基的形成。
Inhibitory effects of flavonoids rutin and quercetin on ferrous ion-dependent lipid peroxidation of lecithin liposomes and NADPH- and CCl4-dependent lipid peroxidation in rat liver microsomes were studied to elucidate the chelating and free radical scavenging activities of these compounds. The interaction of rutin with superoxide ion and ferrous ions and the reaction of quercetin with lipid peroxoy radicals were also studied. Both flavonoids were significantly more effective inhibitors of iron ion-dependent lipid peroxidation systems due to chelating iron ions with the formation of inert iron complexes unable to initiate lipid peroxidation. At the same time, iron complexes of flavonoids retained their free radical scavenging activities. The chelating mechanism of inhibition was more important for rutin than for quercetin. The mutual effect of rutin and ascorbic acid on non-enzymatic lipid peroxidation was also studied. It was concluded that rutin and quercetin are able to suppress free radical processes at three stages: the formation of superoxide ion, the generation of hydroxyl (or cryptohydroxyl) radicals in the Fenton reaction and the formation of lipid peroxy radicals.