Expression of doubly labeled Saccharomyces cerevisiae iso-1 ferricytochrome c and (1)H, (13)C and (15)N chemical shift assignments by multidimensional NMR.
Expression of doubly labeled Saccharomyces cerevisiae iso-1 ferricytochrome c and (1)H, (13)C and (15)N chemical shift assignments by multidimensional NMR.
复制标题
通过多维 NMR 表达双标记酿酒酵母 iso-1 铁细胞色素 c 和 (1)H、(13)C 和 (15)N 化学位移。
DOI：
10.1016/s0014-5793(00)02032-9
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发表时间：
2000
期刊：
影响因子：
3.5
通讯作者：
Oldfield,E
中科院分区：
文献类型：
--
作者：
Szabo,CM;Sanders,LK;Le,HC;Chien,EY;Oldfield,E
We have expressed [U-13C,15N]-labeled Saccharomyces cerevisiae iso-1 cytochrome c C102T;K72A in Escherichia coli with a yield of 11 mg/l of growth medium. Nuclear magnetic resonance (NMR) studies were conducted on the Fe3+form of the protein. We report herein chemical shift assignments for amide1H and15N,13C°,13Cα,13Cβ,1Hαand1Hβresonances based upon a series of three-dimensional NMR experiments: HNCA, HN(CO)CA, HNCO, HN(CA)CO, HNCACB, HCA(CO)N, HCCH-TOCSY and HBHA(CBCA)NH. An investigation of the chemical shifts of the threonine residues was also made by using density functional theory in order to help solve discrepancies between15N chemical shift assignments reported in this study and those reported previously.
