Screening of early antigen genes of adult-stage Trichinella spiralis using pig serum from different stages of early infection
Screening of early antigen genes of adult-stage Trichinella spiralis using pig serum from different stages of early infection
复制标题
利用早期感染不同阶段的猪血清筛选旋毛虫成虫早期抗原基因。
DOI：
10.1016/j.vetpar.2013.02.001
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发表时间：
2013-05-20
影响因子：
2.6
通讯作者：
Liu, M. Y.
中科院分区：
文献类型：
--
作者：
Liu, P.;Wu, X. P.;Liu, M. Y.
这项工作的目标是鉴定旋毛虫中存在的新型早期抗原。为此，使用感染 20,000 只肌肉幼虫的猪的血清对 3 天龄成虫 (Ad3) 生成的 cDNA 文库进行了免疫学筛选。血清是从与早期蠕虫发育相一致的多次、时程采血中获得的：使用感染后 20 天 (dpi) 获得的血清分离出 17 个阳性克隆。所有克隆都对应一个基因，该基因与旋毛虫 ATP 依赖性 RNA 解旋酶 DDX19B 表现出高度序列同一性，该酶参与寄生虫的生长和发育。此外，当用 30 dpi 血清筛选文库时，还鉴定出了代表 5 个独特基因的另外 9 个阳性克隆；这五个基因中的四个与已知参与宿主入侵和宿主-寄生虫相互作用的假定的旋毛虫丝氨酸蛋白酶家族的成员表现出高度相似性。其余基因与 T.spiralis 假设 ORF 11.30 对齐。这些抗原的鉴定为旋毛虫病的早期诊断和针对这种寄生虫的疫苗的开发提供了潜在的候选者。 (C) 2013 Elsevier B.V. 保留所有权利。
The goal of this work was to identify novel, early antigens present in Trichinella spiralis. To this end, a cDNA library generated from 3-day old adult worms (Ad3) was immunologically screened using serum from a pig infected with 20,000 muscle larvae. The serum was obtained from multiple, time course bleeds coinciding with early worm development: Seventeen positive clones were isolated using serum obtained at 20 days post infection (dpi). All clones corresponded to one gene that exhibited high sequence identity with the T. spiralis ATP-dependent RNA helicase DDX19B which is involved in parasite growth and development. In addition, nine additional positive clones representing 5 unique genes were identified when the library was screened with 30 dpi serum; four of these five genes displayed high similarity with members of a putative T. spiralis serine protease family known to be involved in host invasion and host-parasite interactions. The remaining gene aligned with the T. spiralis hypothetical ORF 11.30. The identification of these antigens provides potential candidates for the early diagnosis of trichinellosis and for the development of a vaccine against this parasite. (C) 2013 Elsevier B.V. All rights reserved.