Expression of the tyrosine kinase receptor EphA5 and its ligand ephrin-A5 during mouse spinal cord development.
Expression of the tyrosine kinase receptor EphA5 and its ligand ephrin-A5 during mouse spinal cord development.
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DOI：
10.1007/s12264-007-0037-7
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发表时间：
2007-09-01
影响因子：
5.6
通讯作者：
Zhou, Renping
中科院分区：
文献类型：
--
作者：
Washburn, Christopher P;Cooper, Margaret A;Zhou, Renping
目的：研究Eph家族两种分子EphA 5受体和配体ephrin-A5在脊髓发育过程中的表达模式。用两种不同的亲和探针评估配体的表达，并以敲除小鼠组织作为对照。结果：EphA 5在脊髓腹侧表达，而ephrin-A5在整个发育过程中位于脊髓背外侧区域。这些结果表明EphA 5和ephrin-A5在广泛的发育阶段表达，可能在建立脊髓背腹组织方面发挥重要作用。
OBJECTIVES: To study the expression patterns of two Eph family molecules, the receptor EphA5, and the ligand ephrin-A5, during spinal cord development.METHODS: The receptor expression was analyzed using beta-galactosidase knockin mice, and affinity ligand probe binding. The ligand expression was assessed using two different affinity probes, and knockout mouse tissues as controls.RESULTS: EphA5 was expressed in the ventral spinal cord, while ephrin-A5 was located in the dorsolateral regions of the spinal cord throughout development.CONCLUSIONS: These results show that EphA5 and ephrin-A5 are expressed over broad developmental stages and may play important roles in establishing the dorsoventral organization of the spinal cord.