Sex steroid and 1,25-dihydroxyvitamin D3 receptors in human colorectal adenocarcinoma and normal mucosa.
Sex steroid and 1,25-dihydroxyvitamin D3 receptors in human colorectal adenocarcinoma and normal mucosa.
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发表时间：
1991-02
期刊：
影响因子：
11.2
通讯作者：
F. Meggouh;Patrice Lointier;Simone Saez
中科院分区：
文献类型：
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作者：
F. Meggouh;Patrice Lointier;Simone Saez
为了确定性类固醇和1，25-二羟维生素D3在结直肠癌扩散中的潜在作用，以前从流行病学和实验数据中假设，特异性雄激素（AR，n = 94），雌激素（ER，n = 60），孕酮（PGR，n = 50）和1，25-二羟维生素D3受体（VDR，n = 111）在人大肠腺癌（AC）中进行了研究，并与正常癌旁粘膜（NM）进行了比较。结合数据的Scatchard分析和竞争研究未揭示正常和肿瘤组织受体的生化行为（亲和力、特异性和沉降系数）之间的任何差异。对于AR和ER，在两类组织中均发现高发生率（NM中分别为92/94和90/94，AC中分别为46/60和40/60），而孕酮的发生率较低（NM中为7/50，AC中为5/50）。而性类固醇受体的发生率不随患者的性别和年龄或肿瘤的位置和组织病理学分级而变化，AC中的VDR发生率（35/111）低于NM（99/111），腺癌中从右半结肠到直肠显著降低。NM和AC中ER和VDR的结合能力相似，而NM中的AR水平显著高于AC。VDRs在某些结直肠肿瘤中的表达提示了可能的临床意义。性类固醇受体的已知功能与其在人类结直肠组织中的存在无关，并且其在癌中的模式不支持先前在啮齿动物中化学诱导的结肠肿瘤的情况下提出的任何假设。
In order to determine the potential role of sex steroid and 1,25-dihydroxyvitamin D3 in the spreading of colorectal cancer, previously hypothesized from epidemiological and experimental data, specific androgen (AR, n = 94), estrogen (ER, n = 60), progesterone (PGR, n = 50), and 1,25-dihydroxyvitamin D3 receptors (VDR, (n = 111) were investigated in human colorectal adenocarcinoma (AC) and compared with the normal adjacent mucosa (NM). Scatchard analysis and competition studies of binding data did not reveal any difference between the biochemical behavior (affinity, specificity, and sedimentation coefficient) of the normal and tumoral tissue receptors. For ARs and ERs, high incidences were found (92 of 94 and 90 of 94 in NM versus 46 of 60 and 40 of 60 in AC, respectively) in both classes of tissues, while they were low for progesterone (7 of 50 and 5 of 50 in NM versus AC). While for sex steroid receptors the incidences did not vary with sex and age of the patients or the location and histopathological grade of the tumor, the VDR incidence was lower in AC (35 of 111) than in NM (99 of 111) and decreased significantly from the right colon to the rectum in adenocarcinoma. Binding capacities were similar in NM and AC for ERs and VDRs, whereas AR levels in NM were significantly higher than in AC. The expression of VDRs in some colorectal tumors suggests a possible clinical significance. No known function for sex steroid receptors is related to their presence in human colorectal tissues and their pattern in carcinoma does not support any hypothesis previously raised in the case of chemically induced colonic tumors in rodents.