Population genetics of the non-native freshwater shrimp Palaemon sinensis (Sollaud, 1911) in Japan based on mitochondrial 16S rRNA sequence analysis
Population genetics of the non-native freshwater shrimp Palaemon sinensis (Sollaud, 1911) in Japan based on mitochondrial 16S rRNA sequence analysis
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DOI：
10.3391/ai.2021.16.4.08
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发表时间：
2021-11-01
影响因子：
1.6
通讯作者：
Saito, Hidetoshi
中科院分区：
文献类型：
--
作者：
Ogasawara, Chogo;Imai, Tadashi;Saito, Hidetoshi
长臂虾科的中华白虾是一种原产于中国的物种，2005 年首次在日本作为非本地物种被发现，此后在 47 个县中的 25 个都报告了这种情况。为了从基因角度阐明中华对虾是作为活鱼饵物种从中国进口到日本的，本研究分析了在日本 7 个地理区域（东北、关东、中部、近畿、中国、四国和九州）收集的 165 个样本和从一家渔具店购买的 18 个从中国进口的样本的线粒体 16S rRNA 基因的部分核苷酸序列。所有序列均被分为 11 个单倍型，其中包括一半以上中华松样本共有的一种广泛存在的单倍型。几乎所有进口标本都具有显性单倍型。分子方差分析显示，日本本地和进口样本之间或日本各地区之间没有遗传差异。总体结果表明，中华原虫是通过人类介导的传播引入的；此外，在日本建立的中华松的单倍型分布反映了该物种从中国中部和北部输入的强度。这是首次记录日本非本地中华松树遗传结构的研究。
The palaemonid shrimp Palaemon sinensis, a species native to China, was first discovered as an established non-native species in Japan in 2005, and it has since been reported in 25 of 47 prefectures. To genetically elucidate that P. sinensis was introduced to Japan from China via importation as a live fishing bait species, this study analyzed partial nucleotide sequences of the mitochondrial 16S rRNA gene of 165 specimens collected in 7 geographical regions in Japan (Tohoku, Kanto, Chubu, Kinki, Chugoku, Shikoku and Kyushu), and from 18 imported specimens from China purchased at a fishing store. All sequences were classified into 11 haplotypes, which included one widespread haplotype shared by more than half of the P. sinensis samples. Almost all of the samples of imported specimens possessed the dominant haplotype. Analysis of molecular variance showed no genetic differentiation between the Japanese local and imported samples or among regions in Japan. The overall results suggest that P. sinensis was introduced through human-mediated dispersal; furthermore, the haplotype distributions of P. sinensis established in Japan reflected the intensity of the species' importation from central and northern China. This is the first study to document the genetic structure of non-native P. sinensis in Japan.