ESTABLISHMENT AND CHARACTERIZATION OF PERMANENT MURINE HYBRIDOMAS SECRETING MONOCLONAL ANTI‐THY‐1 ANTIBODIES *
ESTABLISHMENT AND CHARACTERIZATION OF PERMANENT MURINE HYBRIDOMAS SECRETING MONOCLONAL ANTI‐THY‐1 ANTIBODIES *
复制标题
分泌单克隆抗 THY-1 抗体的永久鼠杂交瘤的建立和表征 *
DOI：
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发表时间：
1980
期刊：
影响因子：
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通讯作者：
M. Vojtíšková
中科院分区：
文献类型：
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作者：
P. Dráber;J. Zikán;M. Vojtíšková
通过将小鼠骨髓瘤细胞系P3-NSI/1-Ag 4 - 1（NS-1）的细胞与用C3 H/Di胸腺细胞免疫的AKR/J小鼠的脾细胞融合，随后在组织培养物中生长并选择杂交细胞，制备分泌抗Thy-1抗体的杂交瘤。通过稀释和软琼脂重复克隆细胞，分离出两株分泌IgG 3亚类抗体的永久性杂交瘤1B 5和1aG 4/C5。杂交细胞集落的生长取决于饲养细胞的存在; 1 - 2 X 106/ml的脾细胞最有效，然后是1 - 4 X 106/ml的胸腺细胞和低1 - 2个数量级的腹膜细胞。
Hybridomas secreting anti‐Thy‐1 antibodies were produced by fusing cells of the mouse myeloma line P3‐NSI/1‐Ag4‐1 (NS‐1) with spleen cells from AKR/J mice immunized with C3H/Di thymus cells and by subsequent growth in tissue culture and selection of the hybrid cells. Two permanent hybridomas, 1B5 and 1aG4/C5, secreting antibodies of IgG3 subclass were isolated by repeated cloning of cells by dilution and in soft agar. Growth of the hybrid cell colonies depended on the presence of feeder cells; spleen cells at 1‐2 X 106/ml were most effective, then thymus cells at 1‐4 X 106/ml and peritoneal cells at a concentration 1‐2 orders of magnitude lower.