Icariin inhibits the osteoclast formation induced by RANKL and macrophage-colony stimulating factor in mouse bone marrow culture
Icariin inhibits the osteoclast formation induced by RANKL and macrophage-colony stimulating factor in mouse bone marrow culture
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DOI：
10.1691/ph.2007.5.6682
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发表时间：
2007-05-01
期刊：
影响因子：
1.6
通讯作者：
Wang, Y.
中科院分区：
文献类型：
--
作者：
Chen, K. M.;Ge, B. F.;Wang, Y.
淫羊藿苷是一种异戊烯化的黄酮醇糖苷，包含在草药淫羊藿中，长期以来一直被用于改善骨折愈合或预防骨质疏松症，因为人们相信该草药具有骨骼强化作用。我们以前已经证明，淫羊藿苷促进大鼠骨髓基质细胞的成骨分化，并部分解释了herb. In本研究中，淫羊藿苷对破骨细胞的生成和骨吸收活性的影响进行了研究在小鼠骨髓培养。结果发现，淫羊藿苷剂量依赖性地抑制形成破骨细胞的造血细胞的生长和分化。10 μ M淫羊藿苷组TRAP（+）多核细胞明显少于对照组。10 μ M淫羊藿苷组骨吸收陷窝形成也明显少于对照组。RT-PCR分析显示，TRAP、RANK和CTR基因表达量明显低于对照组。结果表明，淫羊藿苷具有抑制破骨细胞形成和骨吸收活性的作用，提示淫羊藿苷可能是淫羊藿强骨作用的有效成分。
Icariin is a prenylated flavonol glycoside contained in the herb Epimedium, which has long been used to improve bone fracture healing or prevent osteoporosis because of the belief that the herb has bone-strengthening action. We have previously demonstrated that icariin enhances the osteogenic differentiation of rat bone marrow stromal cells, and partially explained the bone-strengthening mechanism of the herb. In the present study, the effect of icariin on osteoclastogenesis and bone resorption activity was investigated in mouse bone marrow culture. It was found that icariin dose-dependently inhibited the growth and differentiation of hemopoietic cells from which osteoclasts were formed. Far less TRAP(+) multinuclear cells appeared in the 10 mu M icariin group than in the control. The bone resorption pits formed in the 10 mu M icariin group was also significantly less than that of the control. RT-PCR analysis showed that the gene expression of TRAP, RANK and CTR was obviously lower than that of the control. It can be concluded that icariin has the ability to inhibit the formation and bone resorption activity of osteoclasts, which suggests that icariin should be the effective component for the bone-strengthening action of herb Epimedium.